848501

antibody from BioLegend

Targeting: NRGN RC3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

848501

Provider

BioLegend

Proper citation

BioLegend Cat#848501, RRID:AB_2650691

Product name

Purified anti-Neurogranin, 70-78

Antibody type

Monoclonal

Reactivity

Human, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A16007A

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot of purified anti-Neurogranin antibody (clone A16007A) and isotype-matched IgG2b control, demonstrating antibody specificity towards endogenous neurogranin protein. Lane 1: 20 µg of human brain tissue lysate. Lane 2: 20 µg of human Alzheimer's disease brain tissue lysate. Lanes 3 and 4: 5 µg of rat brain tissue lysate. Lane 5: 50 ng of human recombinant Neurogranin-GST protein. Blots were incubated with 1 µg/mL of primary antibody overnight at 4°C followed by incubation with HRP labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.

Conjugate

Unconjugated