Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX629703 - Provider product page
- Provider
- GeneTex
- Product name
- ATRX antibody [GT4212]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references ATRX represses alternative lengthening of telomeres.
Napier CE, Huschtscha LI, Harvey A, Bower K, Noble JR, Hendrickson EA, Reddel RR
Oncotarget 2015 Jun 30;6(18):16543-58
Oncotarget 2015 Jun 30;6(18):16543-58
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [GT4212] (GTX629703) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATRX antibody [GT4212] detects ATRX protein by Western blot analysis.A. 30 £gg Neuro2A whole cell lysate/extractB. 30 £gg C8D30 whole cell lysate/extractC. 30 £gg BCL-1 whole cell lysate/extract5 % SDS-PAGEATRX antibody [GT4212] (GTX629703) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATRX antibody [GT4212] detects ATRX protein by Western blot analysis.A. 50 µg 293T whole cell lysate/extractB. 50 µg A431 whole cell lysate/extractC. 50 µg HeLa whole cell lysate/extract5 % SDS-PAGEATRX antibody [GT4212] (GTX629703) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [GT4212] (GTX629703) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATRX antibody [GT4212] detects ATRX protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATRX protein stained by ATRX antibody [GT4212] (GTX629703) diluted at 1:500.Blue: Hoechst 33342 staining.