MA5-24609

antibody from Invitrogen Antibodies

Targeting: ATRX JMS, MRX52, RAD54, XH2, XNP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24609

Provider

Invitrogen Antibodies

Product name

ATRX Monoclonal Antibody (CL0537)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AAWAEYEAEK KGLTMRFNIP TGTNLPPVSF NSQTPYIPFN LGALSAMSNQ QLEDLINQGR EKVVEATNSV TAVRIQPLED IISAVWKENM NLSEAQVQAL ALSRQASQEL DVKRREAIYN DVLTKQQMLI SCVQRILMNR R Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 97%.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CL0537

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Molecular Classification of Neuroendocrine Tumors of the Thymus.

Dinter H, Bohnenberger H, Beck J, Bornemann-Kolatzki K, Schütz E, Küffer S, Klein L, Franks TJ, Roden A, Emmert A, Hinterthaner M, Marino M, Brcic L, Popper H, Weis CA, Pelosi G, Marx A, Ströbel P
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2019 Aug;14(8):1472-1483

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATRX in human cell line A-549 using a ATRX Monoclonal Antibody (CL0537) (Product # MA5-24609).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from A-549 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using ATRX Monoclonal Antibody (CL0537) (Product # MA5-24609). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of RAD54 in HeLa cell line using a RAD54 Monoclonal Antibody (Product # MA5-24609), showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of RAD54 in A431 cell line using a RAD54 Monoclonal Antibody (Product # MA5-24609), showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of RAD54 in MCF7 cell line using a RAD54 Monoclonal Antibody (Product # MA5-24609), showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of RAD54 in U251 cell line using a RAD54 Monoclonal Antibody (Product # MA5-24609), showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ATRX in human glioma using ATRX Monoclonal Antibody (CL0537) (Product # MA5-24609) shows strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ATRX in human fallopian tube using ATRX Monoclonal Antibody (CL0537) (Product # MA5-24609) shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ATRX in human testis using ATRX Monoclonal Antibody (CL0537) (Product # MA5-24609) shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts and Leydig cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ATRX in human liver using an ATRX Monoclonal Antibody (CL0537) (Product # MA5-24609) shows no positivity in hepatocytes as expected.