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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-51656 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATRX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AAWAEYEAEK KGLTMRFNIP TGTNLPPVSF NSQTPYIPFN LGALSAMSNQ QLEDLINQGR EKVVEATNSV TAVRIQPLED IISAVWKENM NLSEAQVQAL ALSRQASQEL DVKRREAIYN DVLTKQQMLI SCVQRILMNR R Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 97%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.16 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATRX was performed by loading 25μg/mL of lysate without (lane 1) or with treatment of Dox for 72 hours in reducing sample buffer (20 mM Tris-HCl (pH 7.5) 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 µg/mL leupeptin) onto an 8% polyacrylamide gel. Proteins were transferred to PVDF membrane under semi-dry transfer conditions, constant amperage at 45mA per gel for 90 minutes. Targets were detected at approximately ~100 kDa for HIRA, above 250 for ATRX and between 75 and 100 kDa for DAXX using polyclonal antibodies (Product # PA5-51656 for ATRX, Product # PA5-82362 for DAXX and Product # PA5-97065 for HIRA) at a dilution of 1:1000 in TBST containing 5% non-fat dry milk, followed by incubation with a secondary antibody at a dilution of 1:10,000. Chemiluminescent detection was performed. Data courtesy of Raghuvar Dronamraju and Brian Strahl.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATRX in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a ATRX Polyclonal Antibody (Product # PA5-51656). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ATRX in human cell line A549 using ATRX Polyclonal Antibody (Product # PA5-51656) shows localization to nucleoplasm and nuclear bodies.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATRX in human glioma using an ATRX Polyclonal Antibody (Product # PA5-51656) shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATRX in human cerebral cortex using an ATRX Polyclonal Antibody (Product # PA5-51656) shows moderate to strong nuclear positivity in neurons and glial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATRX in human fallopian tube using an ATRX Polyclonal Antibody (Product # PA5-51656) shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATRX in human testis using an ATRX Polyclonal Antibody (Product # PA5-51656) shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts and Leydig cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- staining of ATRX in human liver using an ATRX Polyclonal Antibody (Product # PA5-51656) shows no positivity in hepatocytes as expected.
