Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- LS-C47555 - Provider product page
- Provider
- LSBio
- Product name
- PAX3 Antibody (aa2-12) LS-C47555
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Horse, Rabbit
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PAX3 antibody (0.3µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Negative Control showing staining of paraffin embedded Human Esophagus, with no primary antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PAX3 antibody (3 ug/ml) staining of paraffin embedded Human Esophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PAX3 antibody (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Data obtained using previous batch.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PAX3 antibody (4.5µg/ml) staining of paraffin embedded Human Esophagus. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.