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- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MAB2457 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Pax3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Pax3 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_000429- Isotype
- IgG
- Antibody clone number
- 274212
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele.
microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
Lineage of origin in rhabdomyosarcoma informs pharmacological response.
Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma.
Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers.
Functional dissection of Pax3 in paraxial mesoderm development and myogenesis.
Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.
Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage.
Sahakyan V, Pozzo E, Duelen R, Deprest J, Sampaolesi M
Stem cells international 2017;2017:6101609
Stem cells international 2017;2017:6101609
microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
Arts N, Cané S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
PloS one 2015;10(4):e0122517
PloS one 2015;10(4):e0122517
Lineage of origin in rhabdomyosarcoma informs pharmacological response.
Abraham J, Nuñez-Álvarez Y, Hettmer S, Carrió E, Chen HI, Nishijo K, Huang ET, Prajapati SI, Walker RL, Davis S, Rebeles J, Wiebush H, McCleish AT, Hampton ST, Bjornson CR, Brack AS, Wagers AJ, Rando TA, Capecchi MR, Marini FC, Ehler BR, Zarzabal LA, Goros MW, Michalek JE, Meltzer PS, Langenau DM, LeGallo RD, Mansoor A, Chen Y, Suelves M, Rubin BP, Keller C
Genes & development 2014 Jul 15;28(14):1578-91
Genes & development 2014 Jul 15;28(14):1578-91
Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma.
Kikuchi K, Hettmer S, Aslam MI, Michalek JE, Laub W, Wilky BA, Loeb DM, Rubin BP, Wagers AJ, Keller C
PLoS genetics 2014 Jan;10(1):e1004107
PLoS genetics 2014 Jan;10(1):e1004107
Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers.
Plouhinec JL, Roche DD, Pegoraro C, Figueiredo AL, Maczkowiak F, Brunet LJ, Milet C, Vert JP, Pollet N, Harland RM, Monsoro-Burq AH
Developmental biology 2014 Feb 15;386(2):461-72
Developmental biology 2014 Feb 15;386(2):461-72
Functional dissection of Pax3 in paraxial mesoderm development and myogenesis.
Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro RC
Stem cells (Dayton, Ohio) 2013 Jan;31(1):59-70
Stem cells (Dayton, Ohio) 2013 Jan;31(1):59-70
Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
Awaya T, Kato T, Mizuno Y, Chang H, Niwa A, Umeda K, Nakahata T, Heike T
PloS one 2012;7(12):e51638
PloS one 2012;7(12):e51638
MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.
Gianakopoulos PJ, Mehta V, Voronova A, Cao Y, Yao Z, Coutu J, Wang X, Waddington MS, Tapscott SJ, Skerjanc IS
The Journal of biological chemistry 2011 Jan 28;286(4):2517-25
The Journal of biological chemistry 2011 Jan 28;286(4):2517-25
Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage.
Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC
Experimental cell research 2008 May 1;314(8):1721-33
Experimental cell research 2008 May 1;314(8):1721-33
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Pax3 in B16-F1 Mouse Cell Line. Pax3 was detected in immersion fixed B16-F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
