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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX108294 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108294, RRID:AB_1949852
- Product name
- Caveolin 2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Interaction of Caveolin-3 and HCN is involved in the pathogenesis of diabetic cystopathy.
Dong X, Song Q, Zhu J, Zhao J, Liu Q, Zhang T, Long Z, Li J, Wu C, Wang Q, Hu X, Damaser M, Li L
Scientific reports 2016 Apr 28;6:24844
Scientific reports 2016 Apr 28;6:24844
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE GTX108294 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody (GTX108294) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Rat tissue extracts (50 £gg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody (GTX108294) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caveolin 2 antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caveolin 2 protein stained by Caveolin 2 antibody (GTX108294) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caveolin 2 antibody immunoprecipitates caveolin 2 protein in IP experiments.IP samples: A549 whole cell extractA. 30 £gg A549 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of caveolin 2 protein by 4 £gg Caveolin 2 antibody (GTX108294)15 % SDS-PAGEThe immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody (GTX108294) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using Caveolin 2(GTX108294) antibody at 1:500 dilution.
