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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-21927 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caveolin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A549, C8D30, Rat muscle. Predicted reactivity: Cat (100%), Pig (100%), Rabbit (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Caveolin 2 Polyclonal Antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Caveolin 2 protein stained by Caveolin 2 Polyclonal Antibody (Product # PA5-21927) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Caveolin 2 Polyclonal Antibody immunoprecipitates caveolin 2 protein in IP experiments. IP samples: A549 whole cell extract. A. 30 µg A549 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of caveolin 2 protein by 4 µg Caveolin 2 Polyclonal Antibody (Product # PA5-21927). 15 % SDS-PAGE. The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 Polyclonal Antibody (Product # PA5-21927) diluted at 1:500.
