- PA5-21927 - Invitrogen Antibodies CAV2 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Caveolin 2 in whole cell extracts (30 µg). Samples was separated by 15% SDS-PAGE and the membrane was probed with Caveolin 2 Polyclonal antibody (Product # PA5-21927) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Caveolin 2 Polyclonal Antibody (Product # PA5-21927). Sample (30 µg of whole cell lysate). Lane A: A549. 12% SDS PAGE. Caveolin 2 Polyclonal Antibody (Product # PA5-21927) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Caveolin 2 Polyclonal Antibody detects Caveolin 2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 Polyclonal Antibody (Product # PA5-21927) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Caveolin 2 Polyclonal Antibody detects Caveolin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 Polyclonal Antibody (Product # PA5-21927) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Caveolin 2 Rabbit Polyclonal Antibody (Product # PA5-21927) and a 18 kDa band corresponding to Caveolin 2 was observed across cell lines and tissues tested along with increase in expression in 3T3-L1 upon differentiation to adipocytes. Membrane enriched extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), 3T3-L1 (Lane 4), 3T3-L1 differentiated to adipocytes (Lane 5), Mouse Heart (Lane 6), Mouse Lung (Lane 7), Mouse Adipose (Lane 8) and Mouse Spleen (Lane 9) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Caveolin 2 Polyclonal Antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Caveolin 2 protein stained by Caveolin 2 Polyclonal Antibody (Product # PA5-21927) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using Caveolin 2 (Product # PA5-21927) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Caveolin 2 Polyclonal Antibody immunoprecipitates caveolin 2 protein in IP experiments. IP samples: A549 whole cell extract. A. 30 µg A549 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of caveolin 2 protein by 4 µg Caveolin 2 Polyclonal Antibody (Product # PA5-21927). 15 % SDS-PAGE. The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 Polyclonal Antibody (Product # PA5-21927) diluted at 1:500.

PA5-21927