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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- PA5-62181 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NHP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IKADPDGPEA QAEACSGERT YQELLVNQNP IAQPLASRRL TRKLYKCIKK AVKQKQIRRG VKEVQKFVNK GE
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NHP2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a NHP2 Polyclonal Antibody (Product # PA5-62181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NHP2 using NHP2 Polyclonal Antibody (Product # PA5-62181) (A) shows similar pattern to an independent NHP2 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NHP2 in human cell line MCF7 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a NHP2 Polyclonal Antibody (Product # PA5-62181).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NHP2 in human liver using NHP2 Polyclonal Antibody (Product # PA5-62181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NHP2 in human testis using NHP2 Polyclonal Antibody (Product # PA5-62181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NHP2 in human kidney using NHP2 Polyclonal Antibody (Product # PA5-62181).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NHP2 in human esophagus using a NHP2 Polyclonal Antibody (Product # PA5-62181) shows nucleolar positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NHP2 in human fallopian tube, kidney, liver and testis using NHP2 Polyclonal Antibody (Product # PA5-62181) (A) shows similar protein distribution across tissues to an independent NHP2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NHP2 in human fallopian tube using NHP2 Polyclonal Antibody (Product # PA5-62181) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NHP2 in human pancreas using NHP2 Polyclonal Antibody (Product # PA5-62181) shows low expression as expected.
