Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
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Validation data
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- Product number
- LS-B3235 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B3235, RRID:AB_2176404
- Product name
- IHC-plus™ PTBP1 Antibody (aa2-14) LS-B3235
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Porcine, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-PTBP1 / PTB Antibody (0.01µg/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-PTBP1 / PTB Antibody (0.03µg/ml) staining of lysates of cell line NIH3T3 (35µg protein in RIPA buffer). Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-PTBP1 / PTB Antibody (0.1µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-PTBP1 / PTB Antibody (0.1-0.3µg/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.