12582-1-AP
antibody from Proteintech Group
Targeting: PTBP1
HNRNP-I, HNRPI, pPTB, PTB, PTB-1, PTB2, PTB3, PTB4
Antibody data
- Antibody Data
- Antigen structure
- References [42]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- 12582-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12582-1-AP, RRID:AB_2176402
- Product name
- PTBP1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PTBP1 antibody (Cat. #12582-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Cerebral furin deficiency causes hydrocephalus in mice.
USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis.
Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS.
LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement.
PICH Activates Cyclin A1 Transcription to Drive S-Phase Progression and Chemoresistance in Gastric Cancer.
Suppression of TGFβR-Smad3 pathway alleviates the syrinx induced by syringomyelia.
N4-acetylcytidine modification of lncRNA CTC-490G23.2 promotes cancer metastasis through interacting with PTBP1 to increase CD44 alternative splicing.
SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers.
A splicing silencer in SMN2 intron 6 is critical in spinal muscular atrophy.
CircRNA MBOAT2 promotes intrahepatic cholangiocarcinoma progression and lipid metabolism reprogramming by stabilizing PTBP1 to facilitate FASN mRNA cytoplasmic export.
Small ankyrin 1 (sANK1) promotes docetaxel resistance in castration-resistant prostate cancer cells by enhancing oxidative phosphorylation.
Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2.
Systematic characterization of cancer transcriptome at transcript resolution.
PTBP1 promotes IRES-mediated translation of cyclin B1 in cancer.
Kaempferol Can Reverse the 5-Fu Resistance of Colorectal Cancer Cells by Inhibiting PKM2-Mediated Glycolysis.
Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina.
LncRNA MEG3 promotes the sensitivity of bortezomib by inhibiting autophagy in multiple myeloma.
Prognostic Value and Biological Functions of RNA Binding Proteins in Stomach Adenocarcinoma.
Linc01513 inhibits the malignant potential of Nasopharyngeal carcinoma by binding to PTBP1.
Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.
Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells.
Hypoxic preconditioning rejuvenates mesenchymal stem cells and enhances neuroprotection following intracerebral hemorrhage via the miR-326-mediated autophagy.
Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.
LncRNA MIR155HG Promotes Temozolomide Resistance by Activating the Wnt/β-Catenin Pathway Via Binding to PTBP1 in Glioma.
Zika virus dysregulates the expression of astrocytic genes involved in neurodevelopment.
p53-targeted lncRNA ST7-AS1 acts as a tumour suppressor by interacting with PTBP1 to suppress the Wnt/β-catenin signalling pathway in glioma.
Abnormal PTBP1 Expression Sustains the Disease Progression of Multiple Myeloma.
hnRNP K Is a Novel Internal Ribosomal Entry Site-Transacting Factor That Negatively Regulates Foot-and-Mouth Disease Virus Translation and Replication and Is Antagonized by Viral 3C Protease.
circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.
Long noncoding RNA ANCR inhibits the differentiation of mesenchymal stem cells toward definitive endoderm by facilitating the association of PTBP1 with ID2.
Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis.
Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.
SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein-RNA interactions with high accuracy.
Identification of novel PANDAR protein interaction partners involved in splicing regulation.
RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.
TRMP, a p53-inducible long noncoding RNA, regulates G1/S cell cycle progression by modulating IRES-dependent p27 translation.
Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.
Activation of the intronic cryptic 5' splice site depends on its distance to the upstream cassette exon.
LncRNA-MIF, a c-Myc-activated long non-coding RNA, suppresses glycolysis by promoting Fbxw7-mediated c-Myc degradation.
HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells.
Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs.
Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes.
Xie S, Xie X, Tang J, Luo B, Chen J, Wen Q, Zhou J, Chen G
Genes & diseases 2024 May;11(3):101009
Genes & diseases 2024 May;11(3):101009
USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis.
Gao S, Wang Y, Xu Y, Liu L, Liu S
Biochimica et biophysica acta. Molecular basis of disease 2024 Mar;1870(3):167011
Biochimica et biophysica acta. Molecular basis of disease 2024 Mar;1870(3):167011
Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS.
Luo J, Liu J, Mou Y, Luo F, Liao Q, Liao Y
The Journal of toxicological sciences 2023;48(6):345-354
The Journal of toxicological sciences 2023;48(6):345-354
LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement.
Zhang C, Wang H, Liu Q, Dai S, Tian G, Wei X, Li X, Zhao L, Shan B
Journal of experimental & clinical cancer research : CR 2023 Sep 23;42(1):246
Journal of experimental & clinical cancer research : CR 2023 Sep 23;42(1):246
PICH Activates Cyclin A1 Transcription to Drive S-Phase Progression and Chemoresistance in Gastric Cancer.
Ye H, Shi W, Yang J, Wang L, Jiang X, Zhao H, Qin L, Qin J, Li L, Cai W, Guan J, Yang H, Zhou H, Yu Z, Sun H, Jiao Z
Cancer research 2023 Nov 15;83(22):3767-3782
Cancer research 2023 Nov 15;83(22):3767-3782
Suppression of TGFβR-Smad3 pathway alleviates the syrinx induced by syringomyelia.
Liu S, Ma L, Qi B, Li Q, Chen Z, Jian F
Cell & bioscience 2023 May 29;13(1):98
Cell & bioscience 2023 May 29;13(1):98
N4-acetylcytidine modification of lncRNA CTC-490G23.2 promotes cancer metastasis through interacting with PTBP1 to increase CD44 alternative splicing.
Yu XM, Li SJ, Yao ZT, Xu JJ, Zheng CC, Liu ZC, Ding PB, Jiang ZL, Wei X, Zhao LP, Shi XY, Li ZG, Xu WW, Li B
Oncogene 2023 Mar;42(14):1101-1116
Oncogene 2023 Mar;42(14):1101-1116
SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers.
Shi L, Shen W, Davis MI, Kong K, Vu P, Saha SK, Adil R, Kreuzer J, Egan R, Lee TD, Greninger P, Shrimp JH, Zhao W, Wei TY, Zhou M, Eccleston J, Sussman J, Manocha U, Weerasekara V, Kondo H, Vijay V, Wu MJ, Kearney SE, Ho J, McClanaghan J, Murchie E, Crowther GS, Patnaik S, Boxer MB, Shen M, Ting DT, Kim WY, Stanger BZ, Deshpande V, Ferrone CR, Benes CH, Haas W, Hall MD, Bardeesy N
Nature cancer 2023 Mar;4(3):365-381
Nature cancer 2023 Mar;4(3):365-381
A splicing silencer in SMN2 intron 6 is critical in spinal muscular atrophy.
Wang L, Ji Y, Chen Y, Bai J, Gao P, Feng P
Human molecular genetics 2023 Mar 6;32(6):971-983
Human molecular genetics 2023 Mar 6;32(6):971-983
CircRNA MBOAT2 promotes intrahepatic cholangiocarcinoma progression and lipid metabolism reprogramming by stabilizing PTBP1 to facilitate FASN mRNA cytoplasmic export.
Yu X, Tong H, Chen J, Tang C, Wang S, Si Y, Wang S, Tang Z
Cell death & disease 2023 Jan 12;14(1):20
Cell death & disease 2023 Jan 12;14(1):20
Small ankyrin 1 (sANK1) promotes docetaxel resistance in castration-resistant prostate cancer cells by enhancing oxidative phosphorylation.
Yang Y, Qin H, Ding M, Ji C, Chen W, Diao W, Yin H, Chen M, Gan W, Guo H
FEBS open bio 2023 Feb;13(2):257-269
FEBS open bio 2023 Feb;13(2):257-269
Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2.
Cui H, Diedrich JK, Wu DC, Lim JJ, Nottingham RM, Moresco JJ, Yates JR 3rd, Blencowe BJ, Lambowitz AM, Schimmel P
Nature cell biology 2023 Apr;25(4):592-603
Nature cell biology 2023 Apr;25(4):592-603
Systematic characterization of cancer transcriptome at transcript resolution.
Hu W, Wu Y, Shi Q, Wu J, Kong D, Wu X, He X, Liu T, Li S
Nature communications 2022 Nov 10;13(1):6803
Nature communications 2022 Nov 10;13(1):6803
PTBP1 promotes IRES-mediated translation of cyclin B1 in cancer.
Fan X, Zhao Z, Ma L, Huang X, Zhan Q, Song Y
Acta biochimica et biophysica Sinica 2022 May 25;54(5):696-707
Acta biochimica et biophysica Sinica 2022 May 25;54(5):696-707
Kaempferol Can Reverse the 5-Fu Resistance of Colorectal Cancer Cells by Inhibiting PKM2-Mediated Glycolysis.
Wu H, Du J, Li C, Li H, Guo H, Li Z
International journal of molecular sciences 2022 Mar 24;23(7)
International journal of molecular sciences 2022 Mar 24;23(7)
Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina.
Hoang T, Kim DW, Appel H, Pannullo NA, Leavey P, Ozawa M, Zheng S, Yu M, Peachey NS, Blackshaw S
Cell reports 2022 Jun 14;39(11):110849
Cell reports 2022 Jun 14;39(11):110849
LncRNA MEG3 promotes the sensitivity of bortezomib by inhibiting autophagy in multiple myeloma.
Zang X, Wang J, Xia Y, Li J, Chen L, Gu Y, Shen X
Leukemia research 2022 Dec;123:106967
Leukemia research 2022 Dec;123:106967
Prognostic Value and Biological Functions of RNA Binding Proteins in Stomach Adenocarcinoma.
Li J, Zhou W, Wei J, Xiao X, An T, Wu W, He Y
OncoTargets and therapy 2021;14:1689-1705
OncoTargets and therapy 2021;14:1689-1705
Linc01513 inhibits the malignant potential of Nasopharyngeal carcinoma by binding to PTBP1.
Wang J, Cai X, Zhang L, Lei D
Journal of Cancer 2021;12(24):7380-7389
Journal of Cancer 2021;12(24):7380-7389
Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.
Chen J, Wu Y, Luo X, Jin D, Zhou W, Ju Z, Wang D, Meng Q, Wang H, Fu X, Xu J, Song Z
Theranostics 2021;11(15):7507-7526
Theranostics 2021;11(15):7507-7526
Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells.
Wu H, Cui M, Li C, Li H, Dai Y, Cui K, Li Z
Journal of agricultural and food chemistry 2021 Mar 17;69(10):3060-3068
Journal of agricultural and food chemistry 2021 Mar 17;69(10):3060-3068
Hypoxic preconditioning rejuvenates mesenchymal stem cells and enhances neuroprotection following intracerebral hemorrhage via the miR-326-mediated autophagy.
Liu J, He J, Ge L, Xiao H, Huang Y, Zeng L, Jiang Z, Lu M, Hu Z
Stem cell research & therapy 2021 Jul 22;12(1):413
Stem cell research & therapy 2021 Jul 22;12(1):413
Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.
Ma Y, Liu S, Gao J, Chen C, Zhang X, Yuan H, Chen Z, Yin X, Sun C, Mao Y, Zhou F, Shao Y, Liu Q, Xu J, Cheng L, Yu D, Li P, Yi P, He J, Geng G, Guo Q, Si Y, Zhao H, Li H, Banes GL, Liu H, Nakamura Y, Kurita R, Huang Y, Wang X, Wang F, Fang G, Engel JD, Shi L, Zhang YE, Yu J
Developmental cell 2021 Feb 22;56(4):478-493.e11
Developmental cell 2021 Feb 22;56(4):478-493.e11
LncRNA MIR155HG Promotes Temozolomide Resistance by Activating the Wnt/β-Catenin Pathway Via Binding to PTBP1 in Glioma.
He X, Sheng J, Yu W, Wang K, Zhu S, Liu Q
Cellular and molecular neurobiology 2021 Aug;41(6):1271-1284
Cellular and molecular neurobiology 2021 Aug;41(6):1271-1284
Zika virus dysregulates the expression of astrocytic genes involved in neurodevelopment.
Shereen MA, Bashir N, Su R, Liu F, Wu K, Luo Z, Wu J
PLoS neglected tropical diseases 2021 Apr;15(4):e0009362
PLoS neglected tropical diseases 2021 Apr;15(4):e0009362
p53-targeted lncRNA ST7-AS1 acts as a tumour suppressor by interacting with PTBP1 to suppress the Wnt/β-catenin signalling pathway in glioma.
Sheng J, He X, Yu W, Chen Y, Long Y, Wang K, Zhu S, Liu Q
Cancer letters 2021 Apr 10;503:54-68
Cancer letters 2021 Apr 10;503:54-68
Abnormal PTBP1 Expression Sustains the Disease Progression of Multiple Myeloma.
Bai H, Chen B
Disease markers 2020;2020:4013658
Disease markers 2020;2020:4013658
hnRNP K Is a Novel Internal Ribosomal Entry Site-Transacting Factor That Negatively Regulates Foot-and-Mouth Disease Virus Translation and Replication and Is Antagonized by Viral 3C Protease.
Liu W, Yang D, Sun C, Wang H, Zhao B, Zhou G, Yu L
Journal of virology 2020 Aug 17;94(17)
Journal of virology 2020 Aug 17;94(17)
circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.
Sun YM, Wang WT, Zeng ZC, Chen TQ, Han C, Pan Q, Huang W, Fang K, Sun LY, Zhou YF, Luo XQ, Luo C, Du X, Chen YQ
Blood 2019 Oct 31;134(18):1533-1546
Blood 2019 Oct 31;134(18):1533-1546
Long noncoding RNA ANCR inhibits the differentiation of mesenchymal stem cells toward definitive endoderm by facilitating the association of PTBP1 with ID2.
Li J, Yang Y, Fan J, Xu H, Fan L, Li H, Zhao RC
Cell death & disease 2019 Jun 24;10(7):492
Cell death & disease 2019 Jun 24;10(7):492
Biosynthetic CircRNA_001160 induced by PTBP1 regulates the permeability of BTB via the CircRNA_001160/miR-195-5p/ETV1 axis.
Li H, Shen S, Ruan X, Liu X, Zheng J, Liu Y, Yang C, Wang D, Liu L, Ma J, Ma T, Wang P, Cai H, Li Z, Zhao L, Xue Y
Cell death & disease 2019 Dec 20;10(12):960
Cell death & disease 2019 Dec 20;10(12):960
Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.
Azam S, Hou S, Zhu B, Wang W, Hao T, Bu X, Khan M, Lei H
RNA biology 2019 Aug;16(8):1001-1009
RNA biology 2019 Aug;16(8):1001-1009
SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein-RNA interactions with high accuracy.
Zhao Y, Zhang Y, Teng Y, Liu K, Liu Y, Li W, Wu L
Nucleic acids research 2019 Apr 8;47(6):e33
Nucleic acids research 2019 Apr 8;47(6):e33
Identification of novel PANDAR protein interaction partners involved in splicing regulation.
Pospiech N, Cibis H, Dietrich L, Müller F, Bange T, Hennig S
Scientific reports 2018 Feb 12;8(1):2798
Scientific reports 2018 Feb 12;8(1):2798
RNA-binding protein DDX1 is responsible for fatty acid-mediated repression of insulin translation.
Li Z, Zhou M, Cai Z, Liu H, Zhong W, Hao Q, Cheng D, Hu X, Hou J, Xu P, Xue Y, Zhou Y, Xu T
Nucleic acids research 2018 Dec 14;46(22):12052-12066
Nucleic acids research 2018 Dec 14;46(22):12052-12066
TRMP, a p53-inducible long noncoding RNA, regulates G1/S cell cycle progression by modulating IRES-dependent p27 translation.
Yang Y, Wang C, Zhao K, Zhang G, Wang D, Mei Y
Cell death & disease 2018 Aug 30;9(9):886
Cell death & disease 2018 Aug 30;9(9):886
Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.
Liang X, Shi H, Yang L, Qiu C, Lin S, Qi Y, Li J, Zhao A, Liu J
British journal of cancer 2017 Mar 28;116(7):903-911
British journal of cancer 2017 Mar 28;116(7):903-911
Activation of the intronic cryptic 5' splice site depends on its distance to the upstream cassette exon.
Liu W, Li X, Liao S, Dou K, Zhang Y
Gene 2017 Jul 1;619:30-36
Gene 2017 Jul 1;619:30-36
LncRNA-MIF, a c-Myc-activated long non-coding RNA, suppresses glycolysis by promoting Fbxw7-mediated c-Myc degradation.
Zhang P, Cao L, Fan P, Mei Y, Wu M
EMBO reports 2016 Aug;17(8):1204-20
EMBO reports 2016 Aug;17(8):1204-20
HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells.
Guo R, Li Y, Ning J, Sun D, Lin L, Liu X
PloS one 2013;8(4):e62729
PloS one 2013;8(4):e62729
Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs.
Markus MA, Marques FZ, Morris BJ
PloS one 2011;6(12):e28926
PloS one 2011;6(12):e28926
Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes.
Gallagher IJ, Scheele C, Keller P, Nielsen AR, Remenyi J, Fischer CP, Roder K, Babraj J, Wahlestedt C, Hutvagner G, Pedersen BK, Timmons JA
Genome medicine 2010 Feb 1;2(2):9
Genome medicine 2010 Feb 1;2(2):9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12582-1-AP(PTBP1 antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A2780 cells, using PTBP1 antibody 12582-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 12582-1-AP(PTBP1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue