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LearnSTJ70425
antibody from St John's Laboratory
Targeting: PTBP1
HNRNP-I, HNRPI, pPTB, PTB, PTB-1, PTB2, PTB3, PTB4
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [5]
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- Product number
- STJ70425 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-PTBP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
DGIVPDIAVGTKR- Epitope
- N-term
- Isotype
- IgG
- Vial size
- 100μg,,
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70425 (0. 1µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70425 (0. 01µg/ml) staining of HeLa (A) , HepG2 (B) , Jurkat (C) and HEK293 (D) nuclear lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70425 (0. 03µg/ml) staining of lysates of cell line NIH3T3 (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70425 (0. 1-0. 3µg/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70425 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
