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antibody from Invitrogen Antibodies
Targeting: PTBP1
HNRNP-I, HNRPI, pPTB, PTB, PTB-1, PTB2, PTB3, PTB4
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- PA5-81297 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human PTBP1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.
Zhou H, Su J, Hu X, Zhou C, Li H, Chen Z, Xiao Q, Wang B, Wu W, Sun Y, Zhou Y, Tang C, Liu F, Wang L, Feng C, Liu M, Li S, Zhang Y, Xu H, Yao H, Shi L, Yang H
Cell 2020 Apr 30;181(3):590-603.e16
Cell 2020 Apr 30;181(3):590-603.e16
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of PTBP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PTBP1 in human breast carcinoma with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PTBP1 in human kidney with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PTBP1 in human placenta with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PTBP1 in mouse kidney with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PTBP1 in mouse small intestine with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PTBP1 in rat kidney with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:2,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTBP1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 4 µL with PTBP1 Polyclonal Antibody (Product # PA5-81297) and 15 µL of 50 % Protein G agarose. Primary antibody: PTBP1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
