Antibody data
- Antibody Data
 - Antigen structure
 - References [3]
 - Comments [0]
 - Validations
 - Immunocytochemistry [1]
 - Immunohistochemistry [1]
 
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Validation data
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- Product number
 - HPA016740 - Provider product page

 - Provider
 - Atlas Antibodies
 - Proper citation
 - Atlas Antibodies Cat#HPA016740, RRID:AB_1855153
 - Product name
 - Anti-PDLIM5
 - Antibody type
 - Polyclonal
 - Description
 - Polyclonal Antibody against Human PDLIM5, Gene description: PDZ and LIM domain 5, Alternative Gene Names: Enh, LIM, Validated applications: ICC, IHC, WB, Uniprot ID: Q96HC4, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
 - Reactivity
 - Human
 - Host
 - Rabbit
 - Conjugate
 - Unconjugated
 - Isotype
 - IgG
 - Vial size
 - 100 µl
 - Concentration
 - 0.1 mg/ml
 - Storage
 - Store at +4°C for short term storage. Long time storage is recommended at -20°C.
 - Handling
 - The antibody solution should be gently mixed before use.
 
Submitted references		Enigma proteins regulate YAP mechanotransduction.
				
PDLIM5 links kidney anion exchanger 1 (kAE1) to ILK and is required for membrane targeting of kAE1
				
The interactome of LIM domain proteins: The contributions of LIM domain proteins to heart failure and heart development
				
		
	
			Elbediwy A, Vanyai H, Diaz-de-la-Loza MD, Frith D, Snijders AP, Thompson BJ
Journal of cell science 2018 Nov 22;131(22)
		Journal of cell science 2018 Nov 22;131(22)
PDLIM5 links kidney anion exchanger 1 (kAE1) to ILK and is required for membrane targeting of kAE1
			Su Y, Hiemstra T, Yan Y, Li J, Karet H, Rosen L, Moreno P, Karet Frankl F
Scientific Reports 2017;7(1)
		Scientific Reports 2017;7(1)
The interactome of LIM domain proteins: The contributions of LIM domain proteins to heart failure and heart development
			Li A, Ponten F, dos Remedios C
PROTEOMICS 2012;12(2):203-225
		PROTEOMICS 2012;12(2):203-225
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					Supportive validation
					
									
				
				- Submitted by
 - Atlas Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
 - Sample type
 - Human
 
							
					Supportive validation
					
									
				
		
		- Submitted by
 - Atlas Antibodies (provider)
 - Enhanced method
 - Orthogonal validation
 - Main image
 
- Experimental details
 - Immunohistochemistry analysis in human heart muscle and testis tissues using HPA016740 antibody. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.
 - Sample type
 - Human
 - Protocol
 - Protocol