Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- ABIN2509153 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Regulatory Associated Protein of MTOR, Complex 1 (RPTOR) antibody
- Antibody type
- Monoclonal
- Antigen
- This RPTOR antibody is generated from a mouse immunized with recombinant protein.
- Description
- This antibody is purified through a protein G column, followed by dialysis against PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 200 μL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of rat and mouse brain lysates: 1. Anti-TRPC4 (extracellular) antibody (ABIN2510954), (1:200). 2. Anti-TRPC4 (extracellular) antibody, preincubated with the control antigen. Expression of TRPC4 in rat brain Immunohistochemical staining of rat third ventricle region using Anti-TRPC4 (extracellular) antibody (ABIN2510954). A. TRPC4 staining (red) is detected along the wall of the third ventricle (horizontal arrow) and astrocytic fibers extending from the wall (vertical arrow). B. Nucleus staining using DAPI as the counterstain (blue). C. Merge images of A and B. Expression of TRPC4 in rat PC12 cells Immunocytochemical staining of intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TRPC4 (extracellular) antibody (ABIN2510954), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merged image of A and B.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of rat and mouse brain lysates: 1. Anti-TRPC4 (extracellular) antibody (ABIN2510954), (1:200). 2. Anti-TRPC4 (extracellular) antibody, preincubated with the control antigen. Expression of TRPC4 in rat brain Immunohistochemical staining of rat third ventricle region using Anti-TRPC4 (extracellular) antibody (ABIN2510954). A. TRPC4 staining (red) is detected along the wall of the third ventricle (horizontal arrow) and astrocytic fibers extending from the wall (vertical arrow). B. Nucleus staining using DAPI as the counterstain (blue). C. Merge images of A and B. Expression of TRPC4 in rat PC12 cells Immunocytochemical staining of intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TRPC4 (extracellular) antibody (ABIN2510954), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merged image of A and B.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence