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- Antibody Data
- Antigen structure
- References [8]
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- Immunohistochemistry [2]
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- Product number
- AF1749 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat ROBO1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat ROBO1 in direct ELISAs and Western blots.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O55005- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord.
Floor-plate-derived netrin-1 is dispensable for commissural axon guidance.
Spontaneous activity regulates Robo1 transcription to mediate a switch in thalamocortical axon growth.
Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain.
Pioneer longitudinal axons navigate using floor plate and Slit/Robo signals.
The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.
Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons.
Gorla M, Santiago C, Chaudhari K, Layman AAK, Oliver PM, Bashaw GJ
Cell reports 2019 Mar 19;26(12):3298-3312.e4
Cell reports 2019 Mar 19;26(12):3298-3312.e4
Floor-plate-derived netrin-1 is dispensable for commissural axon guidance.
Dominici C, Moreno-Bravo JA, Puiggros SR, Rappeneau Q, Rama N, Vieugue P, Bernet A, Mehlen P, Chédotal A
Nature 2017 May 18;545(7654):350-354
Nature 2017 May 18;545(7654):350-354
Spontaneous activity regulates Robo1 transcription to mediate a switch in thalamocortical axon growth.
Mire E, Mezzera C, Leyva-Díaz E, Paternain AV, Squarzoni P, Bluy L, Castillo-Paterna M, López MJ, Peregrín S, Tessier-Lavigne M, Garel S, Galcerán J, Lerma J, López-Bendito G
Nature neuroscience 2012 Jul 8;15(8):1134-43
Nature neuroscience 2012 Jul 8;15(8):1134-43
Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain.
Hernández-Miranda LR, Cariboni A, Faux C, Ruhrberg C, Cho JH, Cloutier JF, Eickholt BJ, Parnavelas JG, Andrews WD
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Apr 20;31(16):6174-87
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Apr 20;31(16):6174-87
Pioneer longitudinal axons navigate using floor plate and Slit/Robo signals.
Farmer WT, Altick AL, Nural HF, Dugan JP, Kidd T, Charron F, Mastick GS
Development (Cambridge, England) 2008 Nov;135(22):3643-53
Development (Cambridge, England) 2008 Nov;135(22):3643-53
The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.
Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG
Developmental biology 2008 Jan 15;313(2):648-58
Developmental biology 2008 Jan 15;313(2):648-58
Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Aug 27;28(35):8698-708
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Aug 27;28(35):8698-708
Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons.
Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chédotal A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 16;28(16):4244-9
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 16;28(16):4244-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ROBO1 in Rat Brain. ROBO1 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1749) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ROBO1 in Rat Embryo. ROBO1 was detected in immersion fixed frozen sections of rat embryo (neural tube) using 5 µg/mL Goat Anti-Rat ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1749) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
