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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- R34076 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ROBO1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ROBO1 antibody is suitable for use in Immunofluorescence/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the ROBO1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HUVEC lysate with ROBO1 antibody at 1ug/ml. Predicted molecular weight (human): ~181kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human U-2 OS cells with ROBO1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human HeLa cells with ROBO1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver tissue with ROBO1 antibody at 2ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney tissue with ROBO1 antibody at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Negative control: IHC staining of FFPE human kidney tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human cortex tissue with ROBO1 antibody at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Negative control: IHC staining of FFPE human cortex tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
