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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ5425 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ROBO1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ROBO1 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ROBO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human HeLa, 3) human 293T, 4) human Jurkat, 5) rat brain and 6) mouse brain tissue lysate with ROBO1 antibody. Expected molecular weight: 181-250 kDa depending on level of glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human A549 cells with ROBO1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ROBO1 antibody.
