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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29917 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ROBO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, MDA-Mb-361, HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1). Predicted reactivity: Mouse (100%), Rat (97%), Zebrafish (80%), Xenopus laevis (95%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.26 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The neurorepellent, Slit2, prevents macrophage lipid loading by inhibiting CD36-dependent binding and internalization of oxidized low-density lipoprotein.
Risk of spontaneous preterm birth and fetal growth associates with fetal SLIT2.
Yusuf B, Mukovozov I, Patel S, Huang YW, Liu GY, Reddy EC, Skrtic M, Glogauer M, Robinson LA
Scientific reports 2021 Feb 11;11(1):3614
Scientific reports 2021 Feb 11;11(1):3614
Risk of spontaneous preterm birth and fetal growth associates with fetal SLIT2.
Tiensuu H, Haapalainen AM, Karjalainen MK, Pasanen A, Huusko JM, Marttila R, Ojaniemi M, Muglia LJ, Hallman M, Rämet M
PLoS genetics 2019 Jun;15(6):e1008107
PLoS genetics 2019 Jun;15(6):e1008107
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ROBO1 in Various whole cell extracts (30 µg). Samples were separated by 5% SDS-PAGE and the membrane was probed with ROBO1 Polyclonal antibody (Product # PA5-29917) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ROBO1 Polyclonal Antibody (Product # PA5-29917). Sample (15 µg of whole cell lysate). Lane A: MDA-Mb-361. Lane B: HCC1937 Lx ( Lentivirus infection, carry contol vector). Lane C: HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1). ROBO1 Polyclonal Antibody (Product # PA5-29917) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ROBO1 was performed by separating 30 µg of HeLa whole cell and membrane extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ROBO1 Polyclonal Antibody (Product # PA5-29917) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ROBO1 Polyclonal Antibody (Product # PA5-29917). Sample (15 µg of whole cell lysate). Lane A: MDA-Mb-361. Lane B: HCC1937 Lx ( Lentivirus infection, carry contol vector). Lane C: HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1). ROBO1 Polyclonal Antibody (Product # PA5-29917) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ROBO1 Polyclonal Antibody detects ROBO1 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ROBO1 stained by ROBO1 Polyclonal Antibody (Product # PA5-29917) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ROBO1 Polyclonal Antibody detects ROBO1 protein at cytosol on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ROBO1 Polyclonal Antibody (Product # PA5-29917) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
