Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX81821 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81821, RRID:AB_11166634
- Product name
- KLF6 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. For short-term storage, store at 4¢X C (up to 10 days). For long-term storage, aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
Submitted references Meningeal SWI/SNF related, matrix-associated, actin-dependent regulator of chromatin, subfamily B member 1 (SMARCB1)-deficient tumours: an emerging group of meningeal tumours.
Krüppel-like factor 5 as potential molecular marker in cervical cancer and the KLF family profile expression.
Dadone B, Fontaine D, Mondot L, Cristofari G, Jouvet A, Godfraind C, Varlet P, Ranchère-Vince D, Coindre JM, Gastaud L, Baudoin C, Peyron AC, Thyss A, Coutts M, Michiels JF, Pedeutour F, Burel-Vandenbos F, RENOP.
Neuropathology and applied neurobiology 2017 Aug;43(5):433-449
Neuropathology and applied neurobiology 2017 Aug;43(5):433-449
Krüppel-like factor 5 as potential molecular marker in cervical cancer and the KLF family profile expression.
Marrero-Rodríguez D, Taniguchi-Ponciano K, Jimenez-Vega F, Romero-Morelos P, Mendoza-Rodríguez M, Mantilla A, Rodriguez-Esquivel M, Hernandez D, Hernandez A, Gomez-Gutierrez G, Muñoz-Hernandez N, la Cruz HA, Vargas-Requena C, Díaz-Hernández C, Serna-Reyna L, Meraz-Rios M, Bandala C, Ortiz-Leon J, Salcedo M
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Nov;35(11):11399-407
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Nov;35(11):11399-407
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of KLF6 Antibody (C-term) (GTX81821) in mouse stomach tissue lysates (35ug/lane). KLF6 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent confocal image of Hela cell stained with KLF6 Antibody (C-term)(GTX81821).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody (C-term)(GTX81821), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of widr cells using KLF6 Antibody (C-term)(bottom histogram)(GTX81821)compared to a negative control cell (top histogram)FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.