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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-15286 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphB4 Monoclonal Antibody (5B8F7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15286 targets EphB4 in IHC and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 5B8F7
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EphB4 using a EphB4 monoclonal antibody (Product # MA5-15286) against a truncated EphB4 recombinant protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of EphB4 was performed using 70% confluent log phase HT-29, A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EphB4 Mouse Monoclonal Antibody (5B8F7) (Product # MA5-15286) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents A549 cells having no expression of EphB4. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization using EphB4 monoclonal antibody (Product # MA5-15286) followed with DAB staining.
