LS-C369547

antibody from LSBio

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C369547

Provider

LSBio

Product name

DDX3 / DDX3X Antibody (aa2-662) LS-C369547

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: DDX3X antibody at 12 ug/ml. Lane 1: NIH3T3 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 73 kDa. Observed band size: 73 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Rat brain tissue, Rat heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: DDX3X antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 72 kDa Observed band size: 74 kDa