Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB3151 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3151, RRID:AB_1574281
- Product name
- DDX3X polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DDX3X.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Crystal structure of conserved domains 1 and 2 of the human DEAD-box helicase DDX3X in complex with the mononucleotide AMP.
Human DEAD-box ATPase DDX3 shows a relaxed nucleoside substrate specificity.
DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.
Högbom M, Collins R, van den Berg S, Jenvert RM, Karlberg T, Kotenyova T, Flores A, Karlsson Hedestam GB, Schiavone LH
Journal of molecular biology 2007 Sep 7;372(1):150-9
Journal of molecular biology 2007 Sep 7;372(1):150-9
Human DEAD-box ATPase DDX3 shows a relaxed nucleoside substrate specificity.
Franca R, Belfiore A, Spadari S, Maga G
Proteins 2007 Jun 1;67(4):1128-37
Proteins 2007 Jun 1;67(4):1128-37
DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.
Ariumi Y, Kuroki M, Abe K, Dansako H, Ikeda M, Wakita T, Kato N
Journal of virology 2007 Dec;81(24):13922-6
Journal of virology 2007 Dec;81(24):13922-6
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of DDX3X polyclonal antibody (Cat # PAB3151) in HeLa cell line lysates (35 ug/lane). DDX3X (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of DDX3X (arrow) using rabbit DDX3X polyclonal antibody (Cat # PAB3151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with DDX3X polyclonal antibody (Cat # PAB3151) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)