LS-C662015

antibody from LSBio

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C662015

Provider

LSBio

Product name

DDX3 / DDX3X Antibody LS-C662015

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of DDX3 using anti-DDX3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate,Lane 3: human MCF-7 whole Cell lysate,Lane 4: human COLO-320 whole Cell lysate,Lane 5: human A549 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDX3 at approximately 73KD. The expected band size for DDX3 is at 73KD.