Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 10-4046 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-DDX3 Antibody
- Antibody type
- Monoclonal
- Description
- DDX3 (DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked) is having a large family of ATP-dependent RNA helicases that involve in several steps of RNA metabolism. Main functions of DDX3 involve transcription, mRNA export and translation, regulation of apoptosis and hepatitis C virus (HCV) replication. DDX3 has two isoforms produced by alternative splicing. The cytoplasmic form is highly phosphorylated in the G1/S. DDX3 is primarily expressed in nucleus during G0 phase and in the cytoplasm during G1/S phase.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant DDX3 p
rotein (amino acids 10-230) was use
d as the immunogen for this antibod
y.- Isotype
- IgG
- Antibody clone number
- ABM27H2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of DDX3. Anti- DDX3 antibody (Clone: ABM27H2) was used at 2 µg/ml on (1) MCF-7,(2) NIH3T3 (3) HEK293 and (4) EL-4 lysates.
- Protocol
- Protocol