MA5-38509

antibody from Invitrogen Antibodies

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38509

Provider

Invitrogen Antibodies

Product name

DDX3 Monoclonal Antibody (3B9E3)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody has been tested in indirect ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3B9E3

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using DDX3 Monoclonal Antibody (Product # MA5-38509) against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDX3 using DDX3 Monoclonal Antibody (Product # MA5-38509) in HEK293 (1) and DDX3X (AA: 518-661)-human IgG-Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDX3 using DDX3 Monoclonal Antibody (Product # MA5-38509) in HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using DDX3 Monoclonal Antibody (Product # MA5-38509) against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDX3 using DDX3 Monoclonal Antibody (Product # MA5-38509) in HEK293 (1) and DDX3X (AA: 518-661)-human IgG-Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of DDX3 in HeLa cells using DDX3 Monoclonal Antibody (Product # MA5-38509). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of DDX3 in HeLa cells using DDX3 Monoclonal Antibody (Product # MA5-38509) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DDX3 in paraffin-embedded cervical cancer tissues using DDX3 Monoclonal Antibody (Product # MA5-38509) and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of DDX3 in paraffin-embedded ovarian cancer tissues using DDX3 Monoclonal Antibody (Product # MA5-38509) and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of DDX3 in HeLa cells using DDX3 Monoclonal Antibody (Product # MA5-38509) (green) and negative control (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of DDX3 in HepG2 cells using DDX3 Monoclonal Antibody (Product # MA5-38509) (green) and negative control (red).