Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-38509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX3 Monoclonal Antibody (3B9E3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B9E3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using DDX3 Monoclonal Antibody (Product # MA5-38509) against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DDX3 using DDX3 Monoclonal Antibody (Product # MA5-38509) in HEK293 (1) and DDX3X (AA: 518-661)-human IgG-Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DDX3 using DDX3 Monoclonal Antibody (Product # MA5-38509) in HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using DDX3 Monoclonal Antibody (Product # MA5-38509) against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DDX3 using DDX3 Monoclonal Antibody (Product # MA5-38509) in HEK293 (1) and DDX3X (AA: 518-661)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of DDX3 in HeLa cells using DDX3 Monoclonal Antibody (Product # MA5-38509). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of DDX3 in HeLa cells using DDX3 Monoclonal Antibody (Product # MA5-38509) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DDX3 in paraffin-embedded cervical cancer tissues using DDX3 Monoclonal Antibody (Product # MA5-38509) and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of DDX3 in paraffin-embedded ovarian cancer tissues using DDX3 Monoclonal Antibody (Product # MA5-38509) and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of DDX3 in HeLa cells using DDX3 Monoclonal Antibody (Product # MA5-38509) (green) and negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of DDX3 in HepG2 cells using DDX3 Monoclonal Antibody (Product # MA5-38509) (green) and negative control (red).