NBP1-85291

antibody from Novus Biologicals

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85291

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85291, RRID:AB_11034160

Product name

Rabbit Polyclonal DDX3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human DDX3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

The X-Linked DDX3X RNA Helicase Dictates Translation Reprogramming and Metastasis in Melanoma.

Phung B, Cieśla M, Sanna A, Guzzi N, Beneventi G, Cao Thi Ngoc P, Lauss M, Cabrita R, Cordero E, Bosch A, Rosengren F, Häkkinen J, Griewank K, Paschen A, Harbst K, Olsson H, Ingvar C, Carneiro A, Tsao H, Schadendorf D, Pietras K, Bellodi C, Jönsson G
Cell reports 2019 Jun 18;27(12):3573-3586.e7

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Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer drivers.

Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P, Mesirov JP, Beroukhim R, Hahn WC
Cancer discovery 2013 Sep;3(9):1044-57

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51

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Tissue profiling of the mammalian central nervous system using human antibody-based proteomics.

Mulder J, Björling E, Jonasson K, Wernérus H, Hober S, Hökfelt T, Uhlén M
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1612-22

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NBP1-85291] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NBP1-85291] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NBP1-85291] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human Cerebral Cortex tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NBP1-85291] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NBP1-85291] - Analysis in human cell line SiHa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX3 Antibody [NBP1-85291] - Analysis: NBP1-85291 (A) shows similar pattern to independent antibody NBP1-85290 (B)

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human appendix shows strong cytoplasmic positivity in reaction center cells in lymphoid tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse medulla shows strong cytoplasmic immunoreactivity in neurons of the facial nucleus.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse thalamus shows positivity in neurons of the anterodorsal thalamic nucleus.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse midbrain shows neuronal positivity in the red nucleus.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebral cortex shows moderate nuclear immunoreactivity in neuronal cell bodies.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear immunoreactivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human hippocampus shows moderate positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse cerebral cortex shows strong positivity in neurons in the parietal association cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse hippocampus shows moderate positivity in a subset of neurons in the brain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human hippocampus shows moderate positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human Fallopian tube shows weak cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear positivity in neurons.