Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-85290 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85290, RRID:AB_11002336
- Product name
- Rabbit Polyclonal DDX3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat DDX3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: DDX3 Antibody [NBP1-85290] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: DDX3 Antibody [NBP1-85290] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: DDX3 Antibody [NBP1-85290] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX3 Antibody [NBP1-85290] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DDX3X antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85290] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in germinal center cells.