HPA001648

antibody from Atlas Antibodies

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA001648

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001648, RRID:AB_1078635

Product name

Anti-DDX3X

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human DDX3X, Gene description: DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked, Alternative Gene Names: DBX, DDX14, DDX3, HLP2, Validated applications: WB, IHC, ICC, Uniprot ID: O00571, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Aberrant cortical development is driven by impaired cell cycle and translational control in a DDX3X syndrome model

Hoye M, Calviello L, Poff A, Ejimogu N, Newman C, Montgomery M, Ou J, Floor S, Silver D
eLife 2022;11

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C9orf72 arginine-rich dipeptide repeat proteins disrupt karyopherin-mediated nuclear import

Hayes L, Duan L, Bowen K, Kalab P, Rothstein J
eLife 2020;9

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Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development

Lennox A, Hoye M, Jiang R, Johnson-Kerner B, Suit L, Venkataramanan S, Sheehan C, Alsina F, Fregeau B, Aldinger K, Moey C, Lobach I, Afenjar A, Babovic-Vuksanovic D, Bézieau S, Blackburn P, Bunt J, Burglen L, Campeau P, Charles P, Chung B, Cogné B, Curry C, D’Agostino M, Di Donato N, Faivre L, Héron D, Innes A, Isidor B, Keren B, Kimball A, Klee E, Kuentz P, Küry S, Martin-Coignard D, Mirzaa G, Mignot C, Miyake N, Matsumoto N, Fujita A, Nava C, Nizon M, Rodriguez D, Blok L, Thauvin-Robinet C, Thevenon J, Vincent M, Ziegler A, Dobyns W, Richards L, Barkovich A, Floor S, Silver D, Sherr E
Neuron 2020;106(3):404-420.e8

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Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS

Kamelgarn M, Chen J, Kuang L, Arenas A, Zhai J, Zhu H, Gal J
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2016;1862(10):2004-2014

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Systematic Interrogation of 3q26 Identifies TLOC1 and SKIL as Cancer Drivers

Hagerstrand D, Tong A, Schumacher S, Ilic N, Shen R, Cheung H, Vazquez F, Shrestha Y, Kim S, Giacomelli A, Rosenbluh J, Schinzel A, Spardy N, Barbie D, Mermel C, Weir B, Garraway L, Tamayo P, Mesirov J, Beroukhim R, Hahn W
Cancer Discovery 2013;3(9):1044-1057

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

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Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics

Mulder J, Björling E, Jonasson K, Wernérus H, Hober S, Hökfelt T, Uhlén M
Molecular & Cellular Proteomics 2009;8(7):1612-1622

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Sample type

Human