PA5-29170

antibody from Invitrogen Antibodies

Targeting: DDX3X DBX, DDX14, DDX3, HLP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29170

Provider

Invitrogen Antibodies

Product name

DDX3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, U87MG, U87-MG Cytoplasm, Human ESC.

Concentration

1.87 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDX3 using 20 µg of A) HeLa and B) HeLa cytoplasm lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a DDX3 polyclonal antibody (Product # PA5-29170) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDX3 was performed by separating 30 µg of U87-MG whole cell and cytoplasm extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DDX3 Polyclonal Antibody (Product # PA5-29170) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DDX3 Polyclonal Antibody (Product # PA5-29170). Sample (20 µg of whole cell lysate). Lane A: human ESC . 7.5% SDS PAGE. DDX3 Polyclonal Antibody (Product # PA5-29170) diluted at 1:2,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DDX3 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DDX3 Polyclonal Antibody (Product # PA5-29170) at a dilution of 1:2000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DDX3 in paraformaldehyde-fixed Human ESC cells using a DDX3 polyclonal antibody (Product # PA5-29170) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DDX3 was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX3 Polyclonal Antibody (Product # PA5-29170) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DDX3 Polyclonal Antibody detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. DDX3 stained by DDX3 Polyclonal Antibody (Product # PA5-29170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.