Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29170 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, U87MG, U87-MG Cytoplasm, Human ESC. Predicted reactivity: Mouse (98%), Xenopus laevis (97%), Pig (100%), Chicken (98%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.87 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX3 using 20 µg of A) HeLa and B) HeLa cytoplasm lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a DDX3 polyclonal antibody (Product # PA5-29170) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DDX3 was performed by separating 30 µg of U87-MG whole cell and cytoplasm extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DDX3 Polyclonal Antibody (Product # PA5-29170) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX3 Polyclonal Antibody (Product # PA5-29170). Sample (20 µg of whole cell lysate). Lane A: human ESC . 7.5% SDS PAGE. DDX3 Polyclonal Antibody (Product # PA5-29170) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DDX3 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a DDX3 Polyclonal Antibody (Product # PA5-29170) at a dilution of 1:2000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDX3 in paraformaldehyde-fixed Human ESC cells using a DDX3 polyclonal antibody (Product # PA5-29170) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DDX3 was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX3 Polyclonal Antibody (Product # PA5-29170) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDX3 Polyclonal Antibody detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. DDX3 stained by DDX3 Polyclonal Antibody (Product # PA5-29170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
