11673-1-AP

antibody from Proteintech Group

Targeting: ARF4 ARF2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11673-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11673-1-AP, RRID:AB_2058463

Product name

ARF4 antibody

Antibody type

Polyclonal

Description

KD/KO validated ARF4 antibody (Cat. #11673-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity.

Wang B, Wan AH, Xu Y, Zhang RX, Zhao BC, Zhao XY, Shi YC, Zhang X, Xue Y, Luo Y, Deng Y, Neely GG, Wan G, Wang QP
Nature communications 2023 May 16;14(1):2241

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A neurodevelopmental disorder associated with an activating de novo missense variant in ARF1.

Ishida M, Otero MG, Freeman C, Sánchez-Lara PA, Guardia CM, Pierson TM, Bonifacino JS
Human molecular genetics 2023 Mar 20;32(7):1162-1174

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GOLPH3 promotes endotoxemia-induced liver and kidney injury through Golgi stress-mediated apoptosis and inflammatory response.

Dusabimana T, Je J, Yun SP, Kim HJ, Kim H, Park SW
Cell death & disease 2023 Jul 21;14(7):458

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The small GTPase ARF3 controls invasion modality and metastasis by regulating N-cadherin levels.

Sandilands E, Freckmann EC, Cumming EM, Román-Fernández A, McGarry L, Anand J, Galbraith L, Mason S, Patel R, Nixon C, Cartwright J, Leung HY, Blyth K, Bryant DM
The Journal of cell biology 2023 Apr 3;222(4)

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A CRISPR/Cas9-mediated screen identifies determinants of early plasma cell differentiation.

Xiong E, Popp O, Salomon C, Mertins P, Kocks C, Rajewsky K, Chu VT
Frontiers in immunology 2022;13:1083119

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Knockdown of Golgi Stress-Responsive Caspase-2 Ameliorates HLD17-Associated AIMP2 Mutant-Mediated Inhibition of Oligodendroglial Cell Morphological Differentiation.

Ochiai A, Sawaguchi S, Memezawa S, Seki Y, Morimoto T, Oizumi H, Ohbuchi K, Yamamoto M, Mizoguchi K, Miyamoto Y, Yamauchi J
Neurochemical research 2022 Sep;47(9):2617-2631

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p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Tan X, Banerjee P, Shi L, Xiao GY, Rodriguez BL, Grzeskowiak CL, Liu X, Yu J, Gibbons DL, Russell WK, Creighton CJ, Kurie JM
Science advances 2021 Jun;7(25)

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microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma.

Fu J, Lou X, Wan S, Zhao X, Chen Z, Zhu M, Guo L, Wu D, Wang S
Oncology reports 2021 Feb;45(2):764-775

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A role for Rab30 in retrograde trafficking and maintenance of endosome-TGN organization.

Zulkefli KL, Mahmoud IS, Williamson NA, Gosavi PK, Houghton FJ, Gleeson PA
Experimental cell research 2021 Feb 15;399(2):112442

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Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production.

Tan JZA, Fourriere L, Wang J, Perez F, Boncompain G, Gleeson PA
Molecular biology of the cell 2020 Jan 1;31(1):27-44

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SCYL1 arginine methylation by PRMT1 is essential for neurite outgrowth via Golgi morphogenesis.

Amano G, Matsuzaki S, Mori Y, Miyoshi K, Han S, Shikada S, Takamura H, Yoshimura T, Katayama T
Molecular biology of the cell 2020 Aug 15;31(18):1963-1973

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The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection.

Moskovskich A, Goldmann U, Kartnig F, Lindinger S, Konecka J, Fiume G, Girardi E, Superti-Furga G
Scientific reports 2019 Jul 18;9(1):10471

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A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.

Howley BV, Link LA, Grelet S, El-Sabban M, Howe PH
Oncogene 2018 Mar;37(10):1308-1325

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Golgi stress-induced transcriptional changes mediated by MAPK signaling and three ETS transcription factors regulate MCL1 splicing.

Baumann J, Ignashkova TI, Chirasani SR, Ramírez-Peinado S, Alborzinia H, Gendarme M, Kuhnigk K, Kramer V, Lindemann RK, Reiling JH
Molecular biology of the cell 2018 Jan 1;29(1):42-52

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Commensal microbiota-induced microRNA modulates intestinal epithelial permeability through the small GTPase ARF4.

Nakata K, Sugi Y, Narabayashi H, Kobayakawa T, Nakanishi Y, Tsuda M, Hosono A, Kaminogawa S, Hanazawa S, Takahashi K
The Journal of biological chemistry 2017 Sep 15;292(37):15426-15433

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Secretory stressors induce intracellular death receptor accumulation to control apoptosis.

van Raam BJ, Lacina T, Lindemann RK, Reiling JH
Cell death & disease 2017 Oct 5;8(10):e3069

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The number of the C-terminal transmembrane domains has the potency to specify subcellular localization of Sec22c.

Yamamoto Y, Yurugi C, Sakisaka T
Biochemical and biophysical research communications 2017 May 27;487(2):388-395

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Chlamydia Hijacks ARF GTPases To Coordinate Microtubule Posttranslational Modifications and Golgi Complex Positioning.

Wesolowski J, Weber MM, Nawrotek A, Dooley CA, Calderon M, St Croix CM, Hackstadt T, Cherfils J, Paumet F
mBio 2017 May 2;8(3)

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Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b- and AP4-dependent pathway.

Toh WH, Tan JZ, Zulkefli KL, Houghton FJ, Gleeson PA
Traffic (Copenhagen, Denmark) 2017 Mar;18(3):159-175

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TRAPPC13 modulates autophagy and the response to Golgi stress.

Ramírez-Peinado S, Ignashkova TI, van Raam BJ, Baumann J, Sennott EL, Gendarme M, Lindemann RK, Starnbach MN, Reiling JH
Journal of cell science 2017 Jul 15;130(14):2251-2265

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Cell survival and protein secretion associated with Golgi integrity in response to Golgi stress-inducing agents.

Ignashkova TI, Gendarme M, Peschk K, Eggenweiler HM, Lindemann RK, Reiling JH
Traffic (Copenhagen, Denmark) 2017 Aug;18(8):530-544

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Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis.

Hanai A, Ohgi M, Yagi C, Ueda T, Shin HW, Nakayama K
Journal of biochemistry 2016 Feb;159(2):201-8

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Dengue Virus Uses a Non-Canonical Function of the Host GBF1-Arf-COPI System for Capsid Protein Accumulation on Lipid Droplets.

Iglesias NG, Mondotte JA, Byk LA, De Maio FA, Samsa MM, Alvarez C, Gamarnik AV
Traffic (Copenhagen, Denmark) 2015 Sep;16(9):962-77

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IGF-1 drives chromogranin A secretion via activation of Arf1 in human neuroendocrine tumour cells.

Münzberg C, Höhn K, Krndija D, Maaß U, Bartsch DK, Slater EP, Oswald F, Walther P, Seufferlein T, von Wichert G
Journal of cellular and molecular medicine 2015 May;19(5):948-59

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Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity.

Pasetto M, Antignani A, Ormanoglu P, Buehler E, Guha R, Pastan I, Martin SE, FitzGerald DJ
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 10;112(10):E1135-42

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KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum.

Li MY, Grandadam M, Kwok K, Lagache T, Siu YL, Zhang JS, Sayteng K, Kudelko M, Qin CF, Olivo-Marin JC, Bruzzone R, Wang PG
Cell reports 2015 Mar 10;10(9):1496-1507

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Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Kim H, Xu H, Yao Q, Li W, Huang Q, Outeda P, Cebotaru V, Chiaravalli M, Boletta A, Piontek K, Germino GG, Weinman EJ, Watnick T, Qian F
Nature communications 2014 Nov 18;5:5482

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BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.

Lin S, Zhou C, Neufeld E, Wang YH, Xu SW, Lu L, Wang Y, Liu ZP, Li D, Li C, Chen S, Le K, Huang H, Liu P, Moss J, Vaughan M, Shen X
Arteriosclerosis, thrombosis, and vascular biology 2013 Feb;33(2):e31-8

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A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

Reiling JH, Olive AJ, Sanyal S, Carette JE, Brummelkamp TR, Ploegh HL, Starnbach MN, Sabatini DM
Nature cell biology 2013 Dec;15(12):1473-85

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ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus.

Nakai W, Kondo Y, Saitoh A, Naito T, Nakayama K, Shin HW
Molecular biology of the cell 2013 Aug;24(16):2570-81

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Arf4 determines dentate gyrus-mediated pattern separation by regulating dendritic spine development.

Jain S, Yoon SY, Zhu L, Brodbeck J, Dai J, Walker D, Huang Y
PloS one 2012;7(9):e46340

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ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway.

Kondo Y, Hanai A, Nakai W, Katoh Y, Nakayama K, Shin HW
Cell structure and function 2012;37(2):141-54

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Regulation of ADP-ribosylation factor 4 expression by small leucine zipper protein and involvement in breast cancer cell migration.

Jang SY, Jang SW, Ko J
Cancer letters 2012 Jan 28;314(2):185-97

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Class II ADP-ribosylation factors are required for efficient secretion of dengue viruses.

Kudelko M, Brault JB, Kwok K, Li MY, Pardigon N, Peiris JSM, Bruzzone R, Desprès P, Nal B, Wang PG
The Journal of biological chemistry 2012 Jan 2;287(1):767-777

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GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis.

Takashima K, Saitoh A, Hirose S, Nakai W, Kondo Y, Takasu Y, Kakeya H, Shin HW, Nakayama K
Cell structure and function 2011;36(2):223-35

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Identification of ADP-ribosylation factor 4 as a suppressor of N-(4-hydroxyphenyl)retinamide-induced cell death.

Woo IS, Eun SY, Jang HS, Kang ES, Kim GH, Kim HJ, Lee JH, Chang KC, Kim JH, Han CW, Seo HG
Cancer letters 2009 Apr 8;276(1):53-60

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 11673-1-AP( ARF4 Antibody) at dilution of 1:800

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

rat brain tissue were subjected to SDS PAGE followed by western blot with 11673-1-AP( ARF4 Antibody) at dilution of 1:800

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The ARF4 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ARF4. This antibody recognizes human,mouse,rat antigen. The ARF4 antibody has been validated for the following applications: ELISA, WB, IHC, IP, IF analysis.

Appendix

11673-1-AP.pdf

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Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ARF4 (IP:11673-1-AP, 3ug; Detection:11673-1-AP 1:600) with mouse brain tissue lysate 5200ug.

Sample type

tissue