PA5-116155

antibody from Invitrogen Antibodies

Targeting: PLPP4 DPPL2, PPAPDC1, PPAPDC1A

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-116155 - Provider product page
Provider: Invitrogen Antibodies
Product name: PPAPDC1A Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 400 µL
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PLPP4 protein structure - PA5-116155 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8 PLPP4 promoted the proliferation, migration, and invasion of glioma LN229 cells. (A) Representative image of immunohistochemical analysis of paraffin-embedded primary glioma tissue specimens (enlarged X400). PLPP4 staining was stronger in glioma tissues than in normal tissues with a scale of 20mum. (B) PLPP4 expression in different glioma cell lines. (C) Real-time PCR assay was used to detect the expression of PLPP4 (vector, over-expression, shRNA-con, shRNA-PLPP4) in each group of LN229 cells. (D) Western blot analysis was performed to detect PLPP4 expression (vector, over-expression, shRNA-con, shRNA-PLPP4) in each group of LN229 cells. (E) CCK-8 assay (at 1, 2, 3, 4, 5 days) in LN229cells. PLPP4-interference inhibited the proliferation of LN229 cells. (F) Silencing PLPP4 reduced the mean colony number according to the colony formation assay. (G) The migration of LN229 cells in each group was detected by the scratch method. (H) In invasion assay, representative images of LN229 cells (vector, over-expression, shRNA-con, shRNA-PLPP4) (x100). Each bar represents the mean values +- SEM of three independent experiments. Compared to vector, * P < 0.05, ** P < 0.01; compared to shRNA-Con, # P < 0.05, ## P < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 11 Silencing PLPP4 decreased the proliferation, migration, and invasion of pancreatic cancer cells. (A) Expression of PLPP4 protein in pancreatic cancer, a representative image of immunohistochemical analysis of paraffin-embedded specimens from 65 cases of primary pancreatic cancer. (B) Western blot was used to detect the expression of PLPP4 protein in different pancreatic cancer cell lines. (C) The real-time PCR assay was used to detect the expression of PLPP4(vector, over-expression, shRNA-con, shRNA-PLPP4) in PANC-1 cells. (D) Western blot analysis of PLPP4 expression in PANC-1 cells of pancreatic cancer. (E) PANC-1 cells proliferation was analyzed with the CCK-8 assay at 1, 2, 3, 4, 5 days. (F) Silencing PLPP4 reduced the mean colony number in PANC-1 cells according to the colony formation assay. (G) shRNA-PLPP4 or over-expression PLPP4 was transfected into PANC-1 cells, and the migration of cells in each group was detected by the scratch method. (H) Invasion assay was used to detect the invasion of PANC-1 cells. Each bar represents the mean values +- SEM of three independent experiments. Compared to vector, * P < 0.05, ** P < 0.01; compared to shRNA-Con, # P < 0.05, ## P < 0.01.