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- Product number
- PA5-20864 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPAPDC1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human brain tissue lysate. PA5-20864 can be used with blocking peptide PEP-0978.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Extracellular Vesicles Carry lncRNA SNHG16 to Promote Metastasis of Breast Cancer Cells via the miR-892b/PPAPDC1A Axis.
Xia W, Liu Y, Cheng T, Xu T, Dong M, Hu X
Frontiers in cell and developmental biology 2021;9:628573
Frontiers in cell and developmental biology 2021;9:628573
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- FIGURE 6 SNHG16 carried by EVs promoted migration and invasion BC cells by inhibiting miR-892b and upregulating PPAPDC1A. MDA-MB-157 and HS578T cells were transfected with miR-892b-mimic or si-PPAPDC1A, followed by MDA-MB-231 cell-EVs treatment. (A) The miR-892b and PPAPDC1A expression in MDA-MB-157 and HS578T cells was detected using RT-qPCR. (B) The PPAPDC1A protein level in MDA-MB-157 and HS578T cells was detected using Western blotting. (C,D) The migration and invasion of MDA-MB-157 and HS578T cells were measured using Transwell assays. MDA-MB-157 cells and HS578T cells were treated with pcDNA3.1-SNHG16 and miR-892b-mimic. (E) PPAPDC1A mRNA expression in cells was detected using RT-qPCR. (F) PPAPDC1A protein level in cells was detected using Western blotting. Cell experiment was repeated three times. Data were presented as mean +- standard deviation. Data in A and B were analyzed using one-way ANOVA, and data in C and D were analyzed using two-way ANOVA, followed by Tukey's multiple comparison test, * p < 0.05 vs. EVs + mimic-NC group, # p < 0.05 vs. EVs + si-NC group; @ p < 0.05.
