PA5-58101

antibody from Invitrogen Antibodies

Targeting: PSTK C10orf89, MGC35392

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-58101

Provider

Invitrogen Antibodies

Product name

PSTK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: LPPETIHLMG RKLEKPNPEK NAWEHNSLTI PSPACASEAS LEVTDLLLTA LENPVKYAED NMEQKDTDRI ICSTN Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 83%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSTK in control (vector only transfected HEK293T lysate) and PSTK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a PSTK Polyclonal Antibody (Product # PA5-58101).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PSTK in human cell line U-251 MG shows positivity in nucleus & actin filaments. Samples were probed using a PSTK Polyclonal Antibody (Product # PA5-58101).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PSTK in human cell line A-431 using a PSTK Polyclonal Antibody (Product # PA5-58101) shows localization to nuclear bodies and actin filaments.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PSTK in human cell line A-431 using a PSTK Polyclonal Antibody (Product # PA5-58101) shows localization to nuclear bodies and actin filaments.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PSTK in human Testis using PSTK Polyclonal Antibody (Product # PA5-58101) shows strong granular cytoplasmic positivity in Leydig cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PSTK in human Fallopian tube using PSTK Polyclonal Antibody (Product # PA5-58101) shows moderate granular cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PSTK in human Pancreas using PSTK Polyclonal Antibody (Product # PA5-58101) shows low expression in exocrine glandular cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PSTK in human Prostate using PSTK Polyclonal Antibody (Product # PA5-58101) shows strong granular cytoplasmic positivity in smooth muscle cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PSTK in human Testis using PSTK Polyclonal Antibody (Product # PA5-58101) shows strong granular cytoplasmic positivity in Leydig cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PSTK in human Pancreas using PSTK Polyclonal Antibody (Product # PA5-58101) shows low expression in exocrine glandular cells as expected.