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LearnPAB20087
antibody from Abnova Corporation
Targeting: UPF3B
HUPF3B, MRX62, MRX82, RENT3B, UPF3BP1, UPF3BP2, UPF3BP3, UPF3X
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB20087 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB20087, RRID:AB_10962259
- Product name
- UPF3B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant UPF3B.
- Antigen sequence
AKKLDKENLSDERASGQSCTLPKRSDSELKDEKPK
RPEDESGRDYREREREYERDQERILRERERLKRQE
EERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGK
KAESTESIGSSEKTEKKEEVVKRDRIRNKDR- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with UPF3B polyclonal antibody (Cat # PAB20087) at 1:250-1:500 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates with UPF3B polyclonal antibody (Cat # PAB20087) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-IILane 3: PC-12
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG with UPF3B polyclonal antibody (Cat # PAB20087) at 1-4 ug/mL dilution shows positivity in cytoplasm.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human placenta with UPF3B polyclonal antibody (Cat # PAB20087) shows strong cytoplasmic positivity in trophoblastic cells at 1:200-1:500 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
