NBP1-83134
antibody from Novus Biologicals
Targeting: UPF3B
HUPF3B, MRX62, MRX82, RENT3B, UPF3BP1, UPF3BP2, UPF3BP3, UPF3X
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-83134 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83134, RRID:AB_11004053
- Product name
- Rabbit Polyclonal UPF3B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat UPF3B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UPF3B Antibody [NBP1-83134] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UPF3B Antibody [NBP1-83134] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UPF3B Antibody [NBP1-83134] - Analysis using Anti-UPF3B antibody NBP1-83134 (A) shows similar pattern to independent antibody NBP2-55617 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UPF3B Antibody [NBP1-83134] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UPF3B Antibody [NBP1-83134] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic and decidua cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UPF3B Antibody [NBP1-83134] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UPF3B Antibody [NBP1-83134] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UPF3B Antibody [NBP1-83134] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UPF3B Antibody [NBP1-83134] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UPF3B Antibody [NBP1-83134] - Staining in human testis and liver tissues using NBP1-83134 antibody. Corresponding UPF3B RNA-seq data are presented for the same tissues.