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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-48341 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUV420H2 Chimeric Recombinant Rabbit Monoclonal Antibody (RAB-C409)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Specificity: This antibody recognizes KMT5C (Histone-lysine N-methyltransferase KMT5C). It binds to a folded domain, amino acids 2-248. KMT5C is a histone methyltransferase that regulates transcription and maintenance of genome integrity.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- RAB-C409
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of SUV420H2 in HEK293 cells using SUV420H2 Chimeric Monoclonal Antibody (Product # MA5-48341). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with SUV420H2 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom SUV420H2 Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was a Rabbit IgG Chimeric Monoclonal Antibody (Product # MA5-47825) followed by staining with Alexa Fluor 488 secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of SUV420H2 in HeLa cells using SUV420H2 Chimeric Monoclonal Antibody (Product # MA5-48341). Cells were fixed using 2% PFA and stained with an isotype control (black), Rabbit IgG Isotype Control (Product # MA5-47825), or SUV420H2 Chimeric Monoclonal Antibody (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AF488 antibody at a dilution of 1:1,000 and cells analyzed using a flow-cytometer.
