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- Product number
- PA5-103851 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total TUBGCP2.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Spatial learning and memory deficits induced by prenatal glucocorticoid exposure depend on hippocampal CRHR1 and CXCL5 signaling in rats.
Zheng Y, Zhang YM, Tang ZS, Du JK, Guo DW, Xu YJ, Sheng H, Lu JQ, Ni X
Journal of neuroinflammation 2021 Apr 2;18(1):85
Journal of neuroinflammation 2021 Apr 2;18(1):85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GCP2 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GCP2 polyclonal antibody (Product # PA5-103851) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GCP2 in rat brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GCP2 polyclonal antibody (Product # PA5-103851) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GCP2 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with GCP2 polyclonal antibody (Product # PA5-103851) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 CXCL5 localization in the hippocampus of rats. The brain tissues were then fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 24 h at 4 degC. Immunofluorescence analysis was applied to show CXCL5 localization in rat hippocampus. a , representative fluorescence images show the co-localization of CXCL5-positive staining and GFAP-positive staining. b , representative fluorescence images show the co-localization of CXCL5-positive staining and MAP2-positive staining. Arrows indicate the cells with double staining
