HPA046377

antibody from Atlas Antibodies

Targeting: IGSF11 BT-IgSF, CT119, Igsf13, MGC35227, VSIG3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA046377

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA046377, RRID:AB_10966195

Product name

Anti-IGSF11

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PPKCSSAKAFHTEISSSDNNTLTSSNAYNSRYWSN
NPKVHRNTESVSHFSDLGQSFSFHSGNANIPSIYA
NGTHLVPGQHKTLVVTANRGSSPQVMSRSNGSVSR
KP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and U-251MG using Anti-IGSF11 antibody. Corresponding IGSF11 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Show more

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A549 shows localization to nucleus, cytosol & cell junctions.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

Sample type

HUMAN