20639-1-AP

antibody from Proteintech Group

Targeting: CHCHD6 CHCM1, MGC13016, Mic25, MICOS25, PPP1R23

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20639-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20639-1-AP, RRID:AB_10697667

Product name

CHCHD6 antibody

Antibody type

Polyclonal

Description

KD/KO validated CHCHD6 antibody (Cat. #20639-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, IP, WB, ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

BRD4-mediated epigenetic regulation of endoplasmic reticulum-mitochondria contact sites is governed by the mitochondrial complex III.

Chen B, Stark DC, Jadhav PV, Lynn-Nguyen TM, Halligan BS, Rossiter NJ, Sindoni N, Shin M, Paulo JA, Chang M, Koo I, Koshkin S, Eyunni S, Ronchi P, Paulsen MT, Morlacchi P, Hanna DA, Lin J, Guerra RM, Pagliarini DJ, Banerjee R, Parolia A, Ljungman ME, Patterson AD, Mancias JD, Mosalaganti S, Sexton JZ, Calì T, Lyssiotis CA, Shah YM
bioRxiv : the preprint server for biology 2024 Nov 5;

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The role of the mitochondrial outer membrane protein SLC25A46 in mitochondrial fission and fusion.

Schuettpelz J, Janer A, Antonicka H, Shoubridge EA
Life science alliance 2023 Jun;6(6)

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The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy.

Gok MO, Connor OM, Wang X, Menezes CJ, Llamas CB, Mishra P, Friedman JR
The Journal of cell biology 2023 Apr 3;222(4)

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A CHCHD6-APP axis connects amyloid and mitochondrial pathology in Alzheimer's disease.

Shang Y, Sun X, Chen X, Wang Q, Wang EJ, Miller E, Xu R, Pieper AA, Qi X
Acta neuropathologica 2022 Nov;144(5):911-938

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Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes.

Urbach J, Kondadi AK, David C, Naha R, Deinert K, Reichert AS, Anand R
Biochimica et biophysica acta. Biomembranes 2021 Dec 1;1863(12):183683

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MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes.

Anand R, Kondadi AK, Meisterknecht J, Golombek M, Nortmann O, Riedel J, Peifer-Weiß L, Brocke-Ahmadinejad N, Schlütermann D, Stork B, Eichmann TO, Wittig I, Reichert AS
Life science alliance 2020 Oct;3(10)

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Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.

Kondadi AK, Anand R, Hänsch S, Urbach J, Zobel T, Wolf DM, Segawa M, Liesa M, Shirihai OS, Weidtkamp-Peters S, Reichert AS
EMBO reports 2020 Mar 4;21(3):e49776

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QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.

Guarani V, Jardel C, Chrétien D, Lombès A, Bénit P, Labasse C, Lacène E, Bourillon A, Imbard A, Benoist JF, Dorboz I, Gilleron M, Goetzman ES, Gaignard P, Slama A, Elmaleh-Bergès M, Romero NB, Rustin P, Ogier de Baulny H, Paulo JA, Harper JW, Schiff M
eLife 2016 Sep 13;5

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Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Li H, Ruan Y, Zhang K, Jian F, Hu C, Miao L, Gong L, Sun L, Zhang X, Chen S, Chen H, Liu D, Song Z
Cell death and differentiation 2016 Mar;23(3):380-92

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Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.

Ding C, Wu Z, Huang L, Wang Y, Xue J, Chen S, Deng Z, Wang L, Song Z, Chen S
Scientific reports 2015 Nov 4;5:16064

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QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Guarani V, McNeill EM, Paulo JA, Huttlin EL, Fröhlich F, Gygi SP, Van Vactor D, Harper JW
eLife 2015 May 21;4

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20639-1-AP(CHCHD6 antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CHCHD6 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human CHCHD6. This antibody recognizes human antigen. The CHCHD6 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

20639-1-AP.pdf

Show more

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human stomach using 20639-1-AP(CHCHD6 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue