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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-57400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEFSEC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AKFHITVGHE TVMGRLMFFS PAPDNFDQEP ILDSFNFSQE YLFQEQYLSK DLTPAVTDND EADKKAGQAT EGHCPRQQWA LVEFEKPVTC Highest antigen sequence identity to the following orthologs: Mouse - 78%, Rat - 80%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EEFSEC in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using an EEFSEC Polyclonal Antibody (Product # PA5-57400).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of EEFSEC in human cell line U-2 OS shows positivity in nucleus. Samples were probed using an EEFSEC Polyclonal Antibody (Product # PA5-57400).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of EEFSEC in human cell line A-431 using an EEFSEC Polyclonal Antibody (Product # PA5-57400) shows localization to nucleus, nuclear bodies and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EEFSEC in human kidney tissue shows moderate cytoplasmic and nuclear positivity in cells in tubules. Samples were probed using an EEFSEC Polyclonal Antibody (Product # PA5-57400).
