PA5-100756

antibody from Invitrogen Antibodies

Targeting: HIVEP2 HIV-EP2, MBP-2, MIBP1, Schnurri-2, ZAS2, ZNF40B

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-100756

Provider

Invitrogen Antibodies

Product name

HIVEP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total MIBP1.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

EGFR/SRC/ERK-stabilized YTHDF2 promotes cholesterol dysregulation and invasive growth of glioblastoma.

Fang R, Chen X, Zhang S, Shi H, Ye Y, Shi H, Zou Z, Li P, Guo Q, Ma L, He C, Huang S
Nature communications 2021 Jan 8;12(1):177

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 YTHDF2 downregulates LXRA and HIVEP2 through m 6 A-dependent mRNA decay. a Western blotting of LXRalpha and HIVEP2 in GSC11 cells stably transfected with control shRNA or two YTHDF2 shRNAs. b Western blotting of LXRalpha and HIVEP2 in LN229/EGFRvIII tet-on cells cultured with vehicle or Doxycycline and transfected with control siRNA or two individual YTHDF2 siRNAs. c , d MeRIP-seq of GSC11 cells shows m 6 A peaks at individual mRNAs of LXRA and HIVEP2 . The y -axis shows normalized reads distribution for input (blue) and m 6 A IP (red) along the transcripts. The schematic representation of mRNA of LXRA or HIVEP2 (top panel). e , f Lifetime of LXRA ( e ) and HIVEP2 ( f ) mRNA in GSC11 cells expressing shYTHDF2 (shDF2), or shControl (shCtrl). Transcription was inhibited by actinomycin D. g CLIP-qPCR showing the association of LXRA and HIVEP2 transcripts with wild-type (WT) or m 6 A recognition defective (MUT) Flag-YTHDF2 in LN229 cells. Data are mean +- S.E.M., n = 4 biologically independent experiments. h mRNA levels of YTHDF2 , LXRA , and HIVEP2 in LN229 cells transfected with vector, wild type (WT) or m 6 A recognition defective (MUT) YTHDF2. Data are mean +- S.E.M., n = 3 biologically independent experiments. i Western blotting of YTHDF2, LXRalpha, and HIVEP2 in LN229 cells transfected with vector, wild-type (WT) or recognition defective (MUT) YTHDF2. j , k Lifetime of LXRA ( j ) or HIVEP2 ( k ) mRNA in LN229 cells expressing wild-type (WT) or m 6 A recognition defec

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 LXRalpha and HIVEP2 are functionally essential targets of YTHDF2 in cell proliferation, invasion, cholesterol dysregulation, and tumorigenesis of GBM cells. a Western blotting of YTHDF2, LXRalpha, and HIVEP2 in GSC11 cells stably expressing shCtrl, shYTHDF2, or shYTHDF2 plus shLXRA or/and shHIVEP2. Representative blot of three independent experiments is shown. b Proliferation of the cells in ( a ) was measured. Data are mean +- S.E.M., n = 6 biologically independent experiments. c In vitro invasion assay for GSC11 cells expressing shCtrl, shYTHDF2, or shYTHDF2 plus shLXRA or/and shHIVEP2. Data are mean +- S.E.M., n = 4 wells examined over three independent experiments. d , e mRNA levels of LXRA and its downstream targets in shCtrl and shYTHDF2 GSC11 cells ( d ) or in LN229 cells expressing vector or YTHDF2 plasmid ( e ). Data are mean +- S.E.M., n = 3 biologically independent experiments (unpaired two-sided t test). f , g Relative cellular cholesterol of GSC11 cells stably expressing shCtrl, shYTHDF2, shLXRA, or shYTHDF2 plus shLXRA ( f ) or LN229 cells expressing vector or YTHDF2 plasmid ( g ). Data are mean +- S.E.M., n = 6 ( f ) or n = 3 ( g ) biologically independent experiments. h , i Quantification of LDL uptake in GSC11 cells expressing shCtrl, shYTHDF2, or shYTHDF2 plus shLXRA ( h ) or in LN229 cells expressing wild-type (WT) or m 6 A recognition defective (MUT) YTHDF2 ( i ). Data are mean +- S.E.M., n = 3 biologically independent experiments. j In vitro invasi