16268-1-AP

antibody from Proteintech Group

Targeting: CEP63 FLJ13386

Provider product page for 16268-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [15]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 16268-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16268-1-AP, RRID:AB_2077079
Product name: CEP63 antibody
Antibody type: Polyclonal
Description: KD/KO validated CEP63 antibody (Cat. #16268-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, Canine and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat, Canine
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CEP63 protein structure - 16268-1-AP shown in red.

Submitted references Molecular basis promoting centriole triplet microtubule assembly.

Takeda Y, Chinen T, Honda S, Takatori S, Okuda S, Yamamoto S, Fukuyama M, Takeuchi K, Tomita T, Hata S, Kitagawa D
Nature communications 2024 Mar 22;15(1):2216

Time-series reconstruction of the molecular architecture of human centriole assembly.

Laporte MH, Gambarotto D, Bertiaux É, Bournonville L, Louvel V, Nunes JM, Borgers S, Hamel V, Guichard P
Cell 2024 Apr 25;187(9):2158-2174.e19

Zika virus alters centrosome organization to suppress the innate immune response.

Kodani A, Knopp KA, Di Lullo E, Retallack H, Kriegstein AR, DeRisi JL, Reiter JF
EMBO reports 2022 Sep 5;23(9):e52211

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.

Ying Z, Wang K, Wu J, Wang M, Yang J, Wang X, Zhou G, Chen H, Xu H, Sze SCW, Gao F, Li C, Sha O
BMC biology 2022 Oct 24;20(1):240

The mechanism of acentrosomal spindle assembly in human oocytes.

Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361

Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle.

Ito KK, Watanabe K, Ishida H, Matsuhashi K, Chinen T, Hata S, Kitagawa D
The Journal of cell biology 2021 Mar 1;220(3)

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.

Holdgaard SG, Cianfanelli V, Pupo E, Lambrughi M, Lubas M, Nielsen JC, Eibes S, Maiani E, Harder LM, Wesch N, Foged MM, Maeda K, Nazio F, de la Ballina LR, Dötsch V, Brech A, Frankel LB, Jäättelä M, Locatelli F, Barisic M, Andersen JS, Bekker-Jensen S, Lund AH, Rogov VV, Papaleo E, Lanzetti L, De Zio D, Cecconi F
Nature communications 2019 Sep 13;10(1):4176

Massive centriole production can occur in the absence of deuterosomes in multiciliated cells.

Mercey O, Levine MS, LoMastro GM, Rostaing P, Brotslaw E, Gomez V, Kumar A, Spassky N, Mitchell BJ, Meunier A, Holland AJ
Nature cell biology 2019 Dec;21(12):1544-1552

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

Mori M, Hazan R, Danielian PS, Mahoney JE, Li H, Lu J, Miller ES, Zhu X, Lees JA, Cardoso WV
Nature communications 2017 Jul 4;8:15857

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Jayaraman D, Kodani A, Gonzalez DM, Mancias JD, Mochida GH, Vagnoni C, Johnson J, Krogan N, Harper JW, Reiter JF, Yu TW, Bae BI, Walsh CA
Neuron 2016 Nov 23;92(4):813-828

Autophagy controls centrosome number by degrading Cep63.

Watanabe Y, Honda S, Konishi A, Arakawa S, Murohashi M, Yamaguchi H, Torii S, Tanabe M, Tanaka S, Warabi E, Shimizu S
Nature communications 2016 Nov 21;7:13508

Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers.

He S, Zhao Z, Yang Y, O'Connell D, Zhang X, Oh S, Ma B, Lee JH, Zhang T, Varghese B, Yip J, Dolatshahi Pirooz S, Li M, Zhang Y, Li GM, Ellen Martin S, Machida K, Liang C
Nature communications 2015 Aug 3;6:7839

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L, Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ, Walsh CA, Reiter JF
eLife 2015 Aug 22;4

Cep63 and cep152 cooperate to ensure centriole duplication.

Brown NJ, Marjanović M, Lüders J, Stracker TH, Costanzo V
PloS one 2013;8(7):e69986

A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.

Zhao Z, Oh S, Li D, Ni D, Pirooz SD, Lee JH, Yang S, Lee JY, Ghozalli I, Costanzo V, Stark JM, Liang C
Developmental cell 2012 May 15;22(5):1001-16

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human placenta tissue were subjected to SDS PAGE followed by western blot with 16268-1-AP(CEP63 antibody) at dilution of 1:800
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using CEP63 antibody 16268-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-CEP63 (IP:16268-1-AP, 3ug; Detection:16268-1-AP 1:500) with HEK-293 cells lysate 1700ug.
Sample type: cell line