PAB28542

antibody from Abnova Corporation

Targeting: NXF5

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28542

Provider

Abnova Corporation

Product name

NXF5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NXF5.

Antigen sequence

TAPYSVKNKLKPGQMEMLKLTMNKRYNVSQQALDL
QNLRFDPDLMGRDIDIILNRRNCMAATLKITERNF
PELLSLNLCNNKLYQLDGLSDITEKAPKVKTLNLS
KNKLESAWELGKVKGLKLEELWLEGNPLCSTFSDQ
S


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with NXF5 polyclonal antibody (Cat # PAB28542) at 1-4 ug/ml shows positivity in nucleus but not nucleoli, cytoplasm and cytoskeleton (actin filaments).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human NXF5 polyclonal antibody (Cat # PAB28542) shows strong nuclear and moderate cytoplasmic positivity in neuronal cells at 1:10-1:20 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)