Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-57049 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GFM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: SYQGELKKGD TIYNTRTRKK VRLQRLARMH ADMMEDVEEV YAGDICALFG IDCASGDTFT DKANSGLSME SIHVPDPVIS I Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Novel compound mutations in the mitochondrial translation elongation factor (TSFM) gene cause severe cardiomyopathy with myocardial fibro-adipose replacement.
Perli E, Pisano A, Glasgow RIC, Carbo M, Hardy SA, Falkous G, He L, Cerbelli B, Pignataro MG, Zacara E, Re F, Della Monica PL, Morea V, Bonnen PE, Taylor RW, d'Amati G, Giordano C
Scientific reports 2019 Mar 25;9(1):5108
Scientific reports 2019 Mar 25;9(1):5108
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GFM1 in human cell line U-251 MG shows positivity in microtubules. Samples were probed using a GFM1 Polyclonal Antibody (Product # PA5-57049).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GFM1 in human placenta and pancreas tissues using GFM1 Polyclonal Antibody (Product # PA5-57049). Corresponding GFM1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GFM1 in human colon, kidney, lymph node and testis using GFM1 Polyclonal Antibody (Product # PA5-57049) (A) shows similar protein distribution across tissues to an independent GFM1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GFM1 in human placenta using GFM1 Polyclonal Antibody (Product # PA5-57049) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GFM1 in human pancreas using GFM1 Polyclonal Antibody (Product # PA5-57049) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GFM1 in human lymph node using GFM1 Polyclonal Antibody (Product # PA5-57049).