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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-31718 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ZNF639 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32. Predicted reactivity: Xenopus laevis (80%), Pig (100%), Chicken (88%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF639 using 30 µg of NT2D1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZNF639 polyclonal antibody (Product # PA5-31718) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZNF639 showing staining in the nucleolus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a ZNF639 polyclonal antibody (Product # PA5-31718) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
