PA5-120614

antibody from Invitrogen Antibodies

Targeting: TBCCD1 FLJ10560

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120614

Provider

Invitrogen Antibodies

Product name

TBCCD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive test controls include: HeLa, U-87MG, Mouse brain, Mouse spleen. Immunogen sequence: RVFQLLPPCE FYVFIIPFEM EGDTTEIPGG LPSVYQKALG QREQKIQIWQ KTVKEAHLTK DQRKQFQVLV ENKFYEWLIN TGHRQQLDSL VPPAAGSKQA AG

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.11 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TBCCD1 in extracts of various cell lines. Samples were incubated with TBCCD1 Polyclonal antibody (Product # PA5-120614) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TBCCD1 in paraffin-embedded human placenta. Samples were incubated with TBCCD1 Polyclonal antibody (Product # PA5-120614) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TBCCD1 in paraffin-embedded mouse kidney. Samples were incubated with TBCCD1 Polyclonal antibody (Product # PA5-120614) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TBCCD1 in paraffin-embedded rat kidney. Samples were incubated with TBCCD1 Polyclonal antibody (Product # PA5-120614) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TBCCD1 in paraffin-embedded mouse kidney. Samples were incubated with TBCCD1 Polyclonal antibody (Product # PA5-120614) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.