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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- 53-9158-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NLGN4X (Neuroligin-4) Monoclonal Antibody (CSTEM30), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This monoclonal antibody CSTEM30 reacts with human Neuroligin-4, X linked, also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X. Applications Reported: This CSTEM30 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. Applications Tested: This CSTEM30 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human IPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.125 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- CSTEM30
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control (Product # 14-4724-82) (left) or 5 µg/mL Anti-Human NLGN4X (Neuroligin-4) Alexa Fluor® 488 (right). Nuclei are stained with DAPI. An iPSC colony (positive staining) and mouse embryonic fibroblasts (negative staining) are in both fields of view.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of a mixture of normal human iPSC and the murine C2C12 cell line with Anti-Human TRA-1-60 (Podocalyxin) PerCP-eFluor® 710 (Product # 46-8863-82) and 0.06 µg of Mouse IgG2a kappa Isotype Control, Alexa Fluor® 488 (Product # 53-4724-82) (left) or 0.06 µg of Anti-Human NLGN4X (Neuroligin-4) Alexa Fluor® 488 (right). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
- Conjugate
- Green dye
