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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- 14-9158-37 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NLGN4X (Neuroligin-4) Monoclonal Antibody (CSTEM30), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This monoclonal antibody CSTEM30 reacts with human Neuroligin-4, X linked, also known as NLGN4X, NLGN4, Neuroligin-4, and Neuroligin 4X. Applications Reported: This CSTEM30 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. (Fluorochrome-conjugated CSTEM30 is recommended for use in flow cytometery.) Applications Tested: This CSTEM30 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human iPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.5 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CSTEM30
- Vial size
- 2 mg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Antagonizing effects of membrane-acting androgens on the eicosanoid receptor OXER1 in prostate cancer.
Kalyvianaki K, Gebhart V, Peroulis N, Panagiotopoulou C, Kiagiadaki F, Pediaditakis I, Aivaliotis M, Moustou E, Tzardi M, Notas G, Castanas E, Kampa M
Scientific reports 2017 Mar 14;7:44418
Scientific reports 2017 Mar 14;7:44418
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control (Product # 14-4724-82) (left) or 5 µg/mL NLGN4X (Neuroligin-4) Monoclonal Antibody (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI. An iPSC colony (positive staining) and mouse embryonic fibroblasts (negative staining) are in both fields of view.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control (Product # 14-4724-82) (left) or 5 µg/mL NLGN4X (Neuroligin-4) Monoclonal Antibody (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI. An iPSC colony (positive staining) and mouse embryonic fibroblasts (negative staining) are in both fields of view.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2a K Isotype Control (Product # 14-4724-82) (left) or 5 µg/mL NLGN4X (Neuroligin-4) Monoclonal Antibody (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI. An iPSC colony (positive staining) and mouse embryonic fibroblasts (negative staining) are in both fields of view.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of a mixture of normal human iPSC and the murine C2C12 cell line with Anti-Human TRA-1-60 (Podocalyxin) PerCP-eFluor® 710 (Product # 46-8863-82) and 0.25 µg of Mouse IgG2a kappa Isotype Control, Purified (Product # 14-4724-82) (left) or 0.25 µg of Anti-Human NLGN4X (Neuroligin-4) Purified (right) followed by F(ab’)2-Goat anti-Mouse IgG PE (Product # 12-4010-82). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
