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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- PA5-23876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CEPT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse, rat and Xenopus based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Metabolic control of T(FH) cells and humoral immunity by phosphatidylethanolamine.
Vacuole membrane protein 1 marks endoplasmic reticulum subdomains enriched in phospholipid synthesizing enzymes and is required for phosphoinositide distribution.
Fu G, Guy CS, Chapman NM, Palacios G, Wei J, Zhou P, Long L, Wang YD, Qian C, Dhungana Y, Huang H, Kc A, Shi H, Rankin S, Brown SA, Johnson A, Wakefield R, Robinson CG, Liu X, Sheyn A, Yu J, Jackowski S, Chi H
Nature 2021 Jul;595(7869):724-729
Nature 2021 Jul;595(7869):724-729
Vacuole membrane protein 1 marks endoplasmic reticulum subdomains enriched in phospholipid synthesizing enzymes and is required for phosphoinositide distribution.
Tábara LC, Vicente JJ, Biazik J, Eskelinen EL, Vincent O, Escalante R
Traffic (Copenhagen, Denmark) 2018 Aug;19(8):624-638
Traffic (Copenhagen, Denmark) 2018 Aug;19(8):624-638
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CEPT1 in Hela whole cell lysate. Samples were incubated with CEPT1 polyclonal antibody (Product # PA5-23876) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CEPT1 in various lysates. Samples were incubated with CEPT1 polyclonal antibody (Product # PA5-23876) using a dilution of 1:4,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: HL-60 whole cell lysate; Lane 2: Hela whole cell lysate; Lane 3: Human cerebellum tissue lysate; Lane 4: SK-BR-3 whole cell lysate; Lane 5: Mouse brain tissue lysate. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CEPT1 in various lysates. Samples were incubated with CEPT1 polyclonal antibody (Product # PA5-23876) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: HL-60 whole cell lysate; Lane 2: Human cerebellum tissue lysate; Lane 3: SK-BR-3 whole cell lysate; Lane 4: Human placenta tissue lysate; Lane 5: Mouse spleen tissue lysate; Lane 6: Mouse brain tissue lysate. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CEPT1 in paraffin-embedded Human tonsil tissue. Samples were incubated with CEPT1 polyclonal antibody (Product # PA5-23876) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CEPT1 in paraffin-embedded Human breast tissue. Samples were incubated with CEPT1 polyclonal antibody (Product # PA5-23876) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CEPT1 in paraffin-embedded Human breast tissue. Samples were incubated with CEPT1 polyclonal antibody (Product # PA5-23876) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.0).
